Kuzmin I V, Franka R, Rupprecht C E
Centers for Disease Control and Prevention, Atlanta, GA, USA.
Dev Biol (Basel). 2008;131:327-37.
Big brown bats (Eptesicus fuscus), either recently captured individuals or survivors from previous experimental infection with Irkut virus (IRKV), were inoculated with West Caucasian bat virus (WCBV), intramuscularly into the masseter (n=7) or neck (n=8) muscles, or orally (n=6). Three bats inoculated into the neck muscles developed rabies and died between days 10 and 18. Viral RNA was detected in a number of tissues but isolation was successful only from the brain. An oral swab of one of these bats was also PCR-positive, but the isolation attempt failed. Brains, salivary glands and swabs from the survivors (six months observation) were negative, as well as all blood pellets collected. Therefore, no suggestions for a carrier state or viremia were obtained. In four surviving bats inoculated in the masseter muscles, WCBV-neutralizing antibodies were detected up to the end of experiment. The absence of antibodies in the three rabid bats may be the result of shorter incubation periods. Bats infected orally neither died nor responded serologically. In the bats previously infected with IRKV, IRKV-neutralizing antibodies were detected as well, up to the end of observation (12 months after IRKV challenge), even if they were not boosted by WCBV inoculation.
大棕蝠（棕蝠），要么是最近捕获的个体，要么是先前感染伊尔库茨克病毒（IRKV）的实验幸存者，通过肌肉注射到咬肌（n = 7）或颈部肌肉（n = 8），或口服（n = 6）的方式接种西高加索蝙蝠病毒（WCBV）。三只接种到颈部肌肉的蝙蝠患狂犬病并在第10至18天之间死亡。在许多组织中检测到病毒RNA，但仅从大脑中成功分离出病毒。其中一只蝙蝠的口腔拭子PCR检测也呈阳性，但病毒分离尝试失败。幸存者（观察六个月）的大脑、唾液腺和拭子均为阴性，所有采集的血球也均为阴性。因此，未发现携带状态或病毒血症的迹象。在四只接种到咬肌的存活蝙蝠中，直到实验结束都检测到了WCBV中和抗体。三只患狂犬病蝙蝠中未检测到抗体可能是潜伏期较短的结果。口服感染的蝙蝠既未死亡，血清学上也无反应。在先前感染IRKV的蝙蝠中，直到观察结束（IRKV攻击后12个月）也检测到了IRKV中和抗体，即使它们未因接种WCBV而增强。
