Roberts M R, Robson F, Foster G D, Draper J, Scott R J
Department of Botany, University of Leicester, U.K.
Plant Mol Biol. 1991 Aug;17(2):295-9. doi: 10.1007/BF00039509.
The I3 cDNA isolated from a library made from 2-4 mm (immature) anthers of Brassica napus shows microspore-specific expression. Homologous transcripts are detected in buds and anthers of male-fertile plants, but not in green tissues, roots, or in cytoplasmic male-sterile buds. High expression of the transcript is limited to microspores entering and undergoing mitosis. The predicted peptide sequence of the cDNA shows an unusual repeated alanine/proline motif at the C-terminus, which may be of importance in the native protein structure.
