来自玉米的一个花粉特异性cDNA克隆的鉴定及其表达
Characterization of a pollen-specific cDNA clone from Zea mays and its expression.
作者信息
Hanson D D, Hamilton D A, Travis J L, Bashe D M, Mascarenhas J P
机构信息
Department of Biological Sciences, State University of New York, Albany 12222.
出版信息
Plant Cell. 1989 Feb;1(2):173-9. doi: 10.1105/tpc.1.2.173.
Abstract
A pollen-specific cDNA clone, Zmc13, has been isolated from a cDNA library constructed to poly(A) RNA from mature maize pollen. The cDNA as shown by primer extension analysis is a full-length copy of the mRNA. The cDNA has been sequenced and is 929 nucleotides in length plus a 47-nucleotide poly(A) tail. Putative polyadenylation signals are identifiable in the 3'-nontranslated region. The mRNA codes for a predicted polypeptide containing 170 amino acid residues and with a molecular mass of 18.3 kilodaltons. The hydropathy profile suggests a possible signal sequence on the amino terminus. A comparison of the nucleotide and deduced amino acid sequence with sequences in data banks has not shown homology to known molecules. In situ hybridizations using RNA probes show that the mRNA is located in the cytoplasm of the vegetative cell of the pollen grain and after germination is distributed throughout the pollen tube cytoplasm.
摘要
从一个以成熟玉米花粉的聚腺苷酸RNA构建的cDNA文库中分离出了一个花粉特异性cDNA克隆Zmc13。引物延伸分析表明该cDNA是mRNA的全长拷贝。该cDNA已被测序，长度为929个核苷酸，加上一个47个核苷酸的聚腺苷酸尾巴。在3'-非翻译区可识别出推定的聚腺苷酸化信号。该mRNA编码一个预测的含有170个氨基酸残基、分子量为18.3千道尔顿的多肽。亲水性图谱表明在氨基末端可能存在信号序列。将核苷酸和推导的氨基酸序列与数据库中的序列进行比较，未发现与已知分子的同源性。使用RNA探针进行的原位杂交表明，该mRNA位于花粉粒营养细胞的细胞质中，萌发后分布于整个花粉管细胞质中。
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本文引用的文献
1
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Genetics. 1986 Jun;113(2):449-67. doi: 10.1093/genetics/113.2.449.
2
Genes expressed in the male gametophyte of flowering plants and their isolation.开花植物雄配子体中表达的基因及其分离
Plant Physiol. 1987 Feb;83(2):442-7. doi: 10.1104/pp.83.2.442.
3
Analysis of the Complexity and Diversity of mRNAs from Pollen and Shoots of Tradescantia.分析花粉和紫露草芽中 mRNA 的复杂性和多样性。
Plant Physiol. 1984 Jul;75(3):865-8. doi: 10.1104/pp.75.3.865.
4
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
5
The primary structure of a plant storage protein: zein.一种植物储存蛋白的一级结构：玉米醇溶蛋白。
Nucleic Acids Res. 1981 Oct 10;9(19):5163-74. doi: 10.1093/nar/9.19.5163.
6
Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.使用不对称RNA探针通过原位杂交检测海胆胚胎中的mRNA。
Dev Biol. 1984 Feb;101(2):485-502. doi: 10.1016/0012-1606(84)90162-3.
7
The complete nucleotide sequence of a legumin gene from pea (Pisum sativum L.).来自豌豆（Pisum sativum L.）的豆球蛋白基因的完整核苷酸序列。
Nucleic Acids Res. 1984 Jun 11;12(11):4493-506. doi: 10.1093/nar/12.11.4493.
8
The primary structures of two leghemoglobin genes from soybean.来自大豆的两个豆血红蛋白基因的一级结构。
Nucleic Acids Res. 1982 Jan 22;10(2):689-701. doi: 10.1093/nar/10.2.689.
9
Processing of the intron-containing thymidylate synthase (td) gene of phage T4 is at the RNA level.噬菌体T4含内含子的胸苷酸合成酶（td）基因的加工发生在RNA水平。
Cell. 1985 Jun;41(2):375-82. doi: 10.1016/s0092-8674(85)80010-6.
10
Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis.高等植物核基因中假定的聚腺苷酸化信号：汇编与分析
Nucleic Acids Res. 1987 Dec 10;15(23):9627-40. doi: 10.1093/nar/15.23.9627.
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