从患乳腺炎奶牛中分离出的负责生物膜形成的基因的分子特征分析
Molecular characterization of genes responsible for biofilm formation in isolated from mastitic cows.
作者信息
Ibrahim Eman Shafeek, Arafa Amany Ahmed, Dorgam Sohad Mohamed, Eid Rasha Hamdy, Atta Nagwa Sayed, El-Dabae Wahid Hussein, Gaber Sadek Eslam
机构信息
Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.
Udder Health and Neonatal Disease, Animal Reproduction Research Institute, Giza, Egypt.
出版信息
Vet World. 2022 Jan;15(1):205-212. doi: 10.14202/vetworld.2022.205-212. Epub 2022 Jan 30.
BACKGROUND AND AIM
Mastitis is considered a significant disease of lactating animals. There are new attitudes for recognizing genes responsible for causing this disease to overcome and change the manipulation of this problem. This study aimed to isolate and identify strains from mastitic bovine animals and detect some specific biofilm-forming genes (, , and biofilm-associated protein [] genes , and ).
MATERIALS AND METHODS
A total of 121 mastitic milk samples were analyzed using biochemical tests (catalase test, oxidative-fermentative test, and coagulase test) and Gram stain. Multiplex polymerase chain reaction was applied to characterize biofilm genes (, and ) in addition to (, , , , and ).
RESULTS
Among the 121 milk samples, 35 staphylococci isolates were derived with an incidence of 28.92% (35/121); among them, 19 are coagulase positive. Ninety percent of the isolates had genes () while gene was not recognized in any isolate. In addition, the incidence of , was 89.5% each. The prevalence of specific groups (, , , and ) was 78.9%, 52.6%, 10.5%, and 15.8%, respectively.
CONCLUSION
This study concluded that has variant mechanisms of pathogenicity to form biofilm devoid of carrying a specific gene.
背景与目的
乳腺炎被认为是泌乳动物的一种重要疾病。对于识别导致该疾病的基因，出现了新的态度，以克服和改变对这一问题的处理方式。本研究旨在从患乳腺炎的牛中分离和鉴定菌株，并检测一些特定的生物膜形成基因（、和生物膜相关蛋白[]基因、和）。
材料与方法
使用生化试验（过氧化氢酶试验、氧化发酵试验和凝固酶试验）和革兰氏染色对总共121份乳腺炎乳样进行分析。除了（、、、和）之外，还应用多重聚合酶链反应来鉴定生物膜基因（、和）。
结果
在121份乳样中，分离出35株葡萄球菌，发生率为28.92%（35/121）；其中19株凝固酶阳性。90%的分离株有基因（），而在任何分离株中均未识别出基因。此外，和的发生率均为89.5%。特定组（、、、和）的流行率分别为78.9%、52.6%、10.5%和15.8%。
结论
本研究得出结论，在不携带特定基因的情况下，具有形成生物膜的不同致病机制。
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