Moller-Larsen A, Andersen H K, Heron I, Sarov I
Intervirology. 1975;6(4-5):249-57. doi: 10.1159/000149479.
Lymphocytes from 19 healthy donors were tested against purified cytomegalovirus (CMV) strain Ad 169, in a lymphocyte-transformation test. The test was performed in microcultures using various preparations and concentrations of antigen. These included, besides purified CMV, CMV-infected cells, herpes simplex virus type 1 antigen, and control antigens. Lymphocytes from CMV-seropositive donors were stimulated by purified virus and infected cells, whereas lymphocytes from seronegative donors did not respond. Similarly, only lymphocytes from herpes-seropositive donors did respond to herpes antigen. With these preparations of antigen the test was found to be sensitive and specific.
在淋巴细胞转化试验中，对来自19名健康供者的淋巴细胞进行了针对纯化巨细胞病毒（CMV）Ad 169株的检测。该试验在微量培养中进行，使用了各种制剂和浓度的抗原。除了纯化的CMV外，这些还包括CMV感染细胞、1型单纯疱疹病毒抗原和对照抗原。来自CMV血清阳性供者的淋巴细胞受到纯化病毒和感染细胞的刺激，而来自血清阴性供者的淋巴细胞则无反应。同样，只有来自疱疹血清阳性供者的淋巴细胞对疱疹抗原产生反应。使用这些抗原制剂，该试验被发现具有敏感性和特异性。
