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不同株巨细胞病毒对淋巴细胞的体外刺激作用。

In vitro stimulation of lymphocytes by different strains of cytomegalovirus.

作者信息

Gärtner L, Wilhelm J A, Czieschnek R

出版信息

Med Microbiol Immunol. 1982;171(1):53-7. doi: 10.1007/BF02122707.

DOI:10.1007/BF02122707
PMID:6289062
Abstract

The in vitro stimulation of lymphocytes from 15 healty adult donors by 5 different stains of CMV resulted in a positive stimulation ratio of lymphocytes from CMV seropositive donors with each of the 5 different strains. Differences in the absolute strength of the response was observed among the 5 strains for each individual, but we concluded that the use of antigen prepared with 1 strain of CMV is sufficient to obtain a positive or negative result. A maximum value for the in vitro lymphocyte stimulation test, however, will only be obtained using different antigen concentrations from several different CMV strains simultaneously.

摘要

用5种不同的巨细胞病毒(CMV)毒株对15名健康成年供者的淋巴细胞进行体外刺激,结果显示,CMV血清反应阳性供者的淋巴细胞对这5种不同毒株均呈现阳性刺激率。对于每个个体而言,在这5种毒株之间观察到了反应绝对强度的差异,但我们得出结论,使用由1种CMV毒株制备的抗原来获得阳性或阴性结果就足够了。然而,只有同时使用来自几种不同CMV毒株的不同抗原浓度,才能获得体外淋巴细胞刺激试验的最大值。

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引用本文的文献

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Enhancive and suppressive effects of cytomegalovirus on human lymphocyte responses in vitro.巨细胞病毒对体外人淋巴细胞反应的增强和抑制作用。
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本文引用的文献

1
Application of different strains for determining IgM antibodies against cytomegalovirus-induced late antigen.应用不同菌株测定抗巨细胞病毒诱导晚期抗原的IgM抗体。
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Comparison of in vitro lymphocyte proliferations induced by cytomegalovirus-infected human fibroblasts and cell-free cytomegalovirus.巨细胞病毒感染的人成纤维细胞和无细胞巨细胞病毒诱导的体外淋巴细胞增殖的比较。
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Infect Immun. 1979 Oct;26(1):217-24. doi: 10.1128/iai.26.1.217-224.1979.
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Med Microbiol Immunol. 1978 Nov 17;166(1-4):81-9. doi: 10.1007/BF02121137.
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Cell-mediated immunity of cytomegalovirus infection in normal subjects and cardiac transplant patients.正常受试者和心脏移植患者巨细胞病毒感染的细胞介导免疫。
J Infect Dis. 1978 May;137(5):541-9. doi: 10.1093/infdis/137.5.541.