Zaitoun A M, Cobden I, al Mardini H, Record C O
Gastroenterology Unit, Royal Victoria Infirmary, Newcastle.
Gut. 1991 Feb;32(2):183-7. doi: 10.1136/gut.32.2.183.
Morphometric measurements were performed on rectal biopsy specimens from 10 normal control subjects and 33 patients with a relapse of distal ulcerative colitis before and after treatment for four weeks in a double blind controlled trial with oral eudragit S coated 5 amino salicylic acid (n = 12) or rectal prednisolone enemas (n = 15). Measurements were assessed using a computer aided measuring system and a counting technique. When untreated patients were compared with the control group there were significant decreases in the area and height of the surface epithelium, in the area of crypt epithelium, and in the ratios of goblet cells to epithelial cells and of surface epithelium to lamina propria. The vascular and lamina propria areas and the number of intraepithelial polymorphs were increased. Treatment with 5 amino salicylic acid and corticosteroids resulted in similar morphological improvements: there was an increase in the area and height of the surface epithelium and the ratios of surface epithelium to lamina propria and of surface to crypt cell height. The ratio of goblet cells to epithelial cells also increased after treatment, while the numbers of polymorphs in the surface and crypt epithelium and lumen decreased. In conclusion, computerised morphometry is valuable for the assessment of the treatment of patients with ulcerative colitis and that in the doses used both treatments were of similar efficacy.
在一项双盲对照试验中，对10名正常对照者以及33名远端溃疡性结肠炎复发患者的直肠活检标本进行了形态学测量。这些患者分别接受口服丙烯酸树脂S包衣的5-氨基水杨酸治疗（n = 12）或直肠用泼尼松龙灌肠治疗（n = 15），治疗为期四周，测量在治疗前后进行。测量采用计算机辅助测量系统和计数技术。将未治疗的患者与对照组比较，可见表面上皮的面积和高度、隐窝上皮的面积、杯状细胞与上皮细胞的比例以及表面上皮与固有层的比例均显著降低。血管和固有层面积以及上皮内多形核细胞数量增加。5-氨基水杨酸和皮质类固醇治疗导致了相似的形态学改善：表面上皮的面积和高度增加，表面上皮与固有层的比例以及表面与隐窝细胞高度的比例增加。治疗后杯状细胞与上皮细胞的比例也增加，而表面和隐窝上皮及管腔内的多形核细胞数量减少。总之，计算机形态测量对于溃疡性结肠炎患者治疗的评估很有价值，并且在所使用的剂量下，两种治疗方法疗效相似。
