de Castro Oliveira Juliana Velasco, de Melo Fernando Lucas, Romano Camila Malta, Iamarino Atila, Rizzi Thais Sampaio, Yeda Fernanda Peres, Hársi Charlotte Marianna, Wolff José Luiz Caldas, de Andrade Zanotto Paolo Marinho
Departamento de Microbiologia, Laboratório de Evolução Molecular e Bioinformática, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Virus Genes. 2008 Oct;37(2):177-84. doi: 10.1007/s11262-008-0253-x. Epub 2008 Jul 22.
ORF 31 is a unique baculovirus gene in the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D). It encodes a putative polypeptide of 369 aa homologous to poly (ADP-ribose) polymerase (PARP) found in the genomes of several organisms. Moreover, we found a phylogenetic association with Group I PARP proteins and a 3D homology model of its conserved PARP C-terminal catalytic domain indicating that had almost an exact spatial superimposition of <1 A with other PARP available structures. The 5' end of ORF 31 mRNA was located at the first nucleotide of a CATT motif at position -27. Using real-time PCR we detected transcripts at 3 h post-infection (p.i.) increasing until 24 h p.i., which coincides with the onset of DNA replication, suggestive of a possible role in DNA metabolism.
开放阅读框31是斜纹夜蛾多核多角体病毒分离株2D（AgMNPV-2D）基因组中一个独特的杆状病毒基因。它编码一个推定的由369个氨基酸组成的多肽，与在几种生物体基因组中发现的聚（ADP-核糖）聚合酶（PARP）同源。此外，我们发现它与第一组PARP蛋白存在系统发育关联，并且其保守的PARP C末端催化结构域的三维同源模型表明，它与其他可用的PARP结构几乎有<1埃的精确空间叠加。开放阅读框31 mRNA的5'端位于-27位CATT基序的第一个核苷酸处。使用实时PCR，我们在感染后3小时（p.i.）检测到转录本，直至感染后24小时转录本增加，这与DNA复制的开始时间一致，提示其在DNA代谢中可能发挥作用。
