von Meltzer Markus, Vásquez Silvia, Sun Jianguang, Wendt Ulrike C, May Anja, Gerlich Wolfram H, Radtke Monika, Schaefer Stephan
Abteilung für Virologie, Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Universität Rostock, Schillingallee 70, D-18057, Rostock, Germany.
Virus Genes. 2008 Oct;37(2):225-30. doi: 10.1007/s11262-008-0261-x. Epub 2008 Jul 23.
There are eight genotypes A-H of hepatitis B virus (HBV). Most genotypes are further divided into subgenotypes. Genotypes and subgenotypes influence the natural course of infection and therapy. We analysed nine sera from HBV carriers from Peru. Using the small hepatitis B surface protein HBs, all samples could be grouped to genotype F. Sequencing of three complete Peruvian genomes showed that HBV from Peru belongs to subgenotype F1. Two of the genomes from HBeAg positive carriers coded surprisingly for a stop codon in the polymerase-ORF leading to a translational stop after 213 and 214 aa, respectively. The third isolate from an HBe Ag positive carrier had three deletions: aa 1-53 and aa 111-142 in preS. In addition nt. 2002-2087 in the HBc-ORF were deleted, leading to an HBc starting at aa 66.
乙型肝炎病毒（HBV）有A - H八种基因型。大多数基因型可进一步分为亚基因型。基因型和亚基因型会影响感染和治疗的自然病程。我们分析了来自秘鲁的9名HBV携带者的血清。使用乙肝表面小蛋白HBs，所有样本都可归为F基因型。对三个完整的秘鲁基因组进行测序表明，秘鲁的HBV属于F1亚基因型。来自HBeAg阳性携带者的两个基因组在聚合酶开放阅读框中令人惊讶地编码了一个终止密码子，分别导致在213和214个氨基酸后发生翻译终止。来自HBeAg阳性携带者的第三个分离株有三个缺失：前S区的1 - 53氨基酸和111 - 142氨基酸。此外，HBc开放阅读框中的2002 - 2087核苷酸被删除，导致HBc从66个氨基酸处开始。
