Hendriks L, Goris A, Van de Peer Y, Neefs J M, Vancanneyt M, Kersters K, Hennebert G L, De Wachter R
Departement Biochemie, Universiteit Antwerpen (UIA), Belgium.
J Gen Microbiol. 1991 May;137(5):1223-30. doi: 10.1099/00221287-137-5-1223.
The classification of species belonging to the genus Candida Berkhout is problematic. Therefore, we have determined the small ribosomal subunit RNA (srRNA) sequences of the type strains of three human pathogenic Candida species; Candida krusei, C. lusitaniae and C. tropicalis. The srRNA sequences were aligned with published eukaryotic srRNA sequences and evolutionary trees were inferred using a matrix optimization method. An evolutionary tree comprising all available eukaryotic srRNA sequences, including two other pathogenic Candida species, C. albicans and C. glabrata, showed that the yeasts diverge rather late in the course of eukaryote evolution, namely at the same depth as green plants, ciliates and some smaller taxa. The cluster of the higher fungi consists of 10 ascomycetes and ascomycete-like species with the first branches leading to Neurospora crassa, Pneumocystis carinii, Candida lusitaniae and C. krusei, in that order. Next there is a dichotomous divergence leading to a group consisting of Torulaspora delbrueckii, Saccharomyces cerevisiae, C. glabrata and Kluyveromyces lactis and a smaller group comprising C. tropicalis and C. albicans. The divergence pattern obtained on the basis of srRNA sequence data is also compared to various other chemotaxonomic data.
伯克霍尔德念珠菌属（Candida Berkhout）物种的分类存在问题。因此，我们测定了三种人类致病念珠菌模式菌株的小核糖体亚基RNA（srRNA）序列，即克鲁斯念珠菌（Candida krusei）、葡萄牙念珠菌（C. lusitaniae）和热带念珠菌（C. tropicalis）。将这些srRNA序列与已发表的真核生物srRNA序列进行比对，并使用矩阵优化方法推断进化树。包含所有可用真核生物srRNA序列（包括另外两种致病念珠菌，白色念珠菌（C. albicans）和光滑念珠菌（C. glabrata））的进化树表明，这些酵母在真核生物进化过程中分歧较晚，即在与绿色植物、纤毛虫和一些较小分类单元相同的深度处。高等真菌的聚类由10个子囊菌和类子囊菌物种组成，其第一个分支依次通向粗糙脉孢菌（Neurospora crassa）、卡氏肺孢子虫（Pneumocystis carinii）、葡萄牙念珠菌和克鲁斯念珠菌。接下来是一个二叉分歧，导致一个由德氏有孢圆酵母（Torulaspora delbrueckii）、酿酒酵母（Saccharomyces cerevisiae）、光滑念珠菌和乳酸克鲁维酵母（Kluyveromyces lactis）组成 的群体，以及一个由热带念珠菌和白色念珠菌组成的较小群体。还将基于srRNA序列数据获得的分歧模式与各种其他化学分类数据进行了比较。
