Chateigner-Boutin Anne-Laure, Ramos-Vega Maricela, Guevara-García Arturo, Andrés Charles, de la Luz Gutiérrez-Nava María, Cantero Araceli, Delannoy Etienne, Jiménez Luis Felipe, Lurin Claire, Small Ian, León Patricia
ARC Centre of Excellence in Plant Energy Biology, Molecular and Chemical Sciences Building (M316), University of Western Australia, 35 Stirling Highway, Crawley, Perth, 6009 WA, Australia.
Plant J. 2008 Nov;56(4):590-602. doi: 10.1111/j.1365-313X.2008.03634.x. Epub 2008 Aug 23.
RNA editing changes the sequence of many transcripts in plant organelles, but little is known about the molecular mechanisms determining the specificity of the process. In this study, we have characterized CLB19 (also known as PDE247), a gene that is required for editing of two distinct chloroplast transcripts, rpoA and clpP. Loss-of-function clb19 mutants present a yellow phenotype with impaired chloroplast development and early seedling lethality under greenhouse conditions. Transcript patterns are profoundly affected in the mutant plants, with a pattern entirely consistent with a defect in activity of the plastid-encoded RNA polymerase. CLB19 encodes a pentatricopeptide repeat protein similar to the editing specificity factors CRR4 and CRR21, but, unlike them, is implicated in editing of two target sites.
RNA编辑改变了植物细胞器中许多转录本的序列，但对于决定该过程特异性的分子机制却知之甚少。在本研究中，我们鉴定了CLB19（也称为PDE247），这是一个编辑两个不同叶绿体转录本rpoA和clpP所必需的基因。功能缺失的clb19突变体呈现黄色表型，叶绿体发育受损，在温室条件下幼苗早期致死。突变植株中的转录本模式受到深刻影响，其模式与质体编码的RNA聚合酶活性缺陷完全一致。CLB19编码一种类似编辑特异性因子CRR4和CRR21的五肽重复蛋白，但与它们不同的是，它参与两个靶位点的编辑。
