Maslova E V
Genetika. 2008 Mar;44(3):366-73.
Two species of the genus Galeopsis L., G. tetrahit L. and G. bifida (family Lamiaceae), are problematic to distinguish often wrongly recognized, and treated by some taxonomists as a single species. Morphological diagnostical characters of these species are variable and partly overlap. Species independence of G. tetrahit and G. bifida was evaluated and their diagnostic characters verified using ISSR and RAPD markers. A total of 57 ISSR and 28 RAPD fragments were obtained providing distinct subdivision of the accessions examined into two groups. Analysis of molecular data using the neighbor-joining method showed that the accessions studied fell into two clades in the same way as demonstrated by the analysis of 20 morphological characters using single linkage method. The morphological characters were found to be more variable compared to the molecular markers, although the combined of these characters provided differentiation of the species.
糙苏属（Galeopsis L.）的两个物种，即四裂糙苏（G. tetrahit L.）和裂叶糙苏（G. bifida，唇形科），很难区分，常常被错误识别，一些分类学家将它们视为单一物种。这些物种的形态诊断特征具有变异性且部分重叠。利用ISSR和RAPD标记评估了四裂糙苏和裂叶糙苏的物种独立性，并验证了它们的诊断特征。共获得57个ISSR片段和28个RAPD片段，将所检测的种质明显细分为两组。使用邻接法对分子数据进行分析表明，所研究的种质分成两个进化枝，这与使用单连锁法对20个形态特征进行分析的结果相同。尽管这些特征的组合能够区分这两个物种，但发现形态特征比分子标记的变异性更大。
