抗视网膜抗体检测和测量标准化的必要性。
The need for standardization of antiretinal antibody detection and measurement.
作者信息
Forooghian Farzin, Macdonald Ian M, Heckenlively John R, Héon Elise, Gordon Lynn K, Hooks John J, Detrick Barbara, Nussenblatt Robert B
机构信息
Division of Epidemiology and Clinical Research, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
Am J Ophthalmol. 2008 Oct;146(4):489-95. doi: 10.1016/j.ajo.2008.05.046. Epub 2008 Jul 30.
PURPOSE
To review the current literature on the detection and measurement of antiretinal antibodies.
DESIGN
Collaborative essay.
METHODS
Literature review and interpretation.
RESULTS
There is strong evidence to suggest a role for antiretinal antibodies, particularly those targeting recoverin and alpha-enolase, in the pathogenesis of autoimmune retinopathy (AIR). Additionally, numerous other autoantibodies have been described as putative mediators of retinal degeneration and more remain to be discovered. However, assay methods described in the literature by many laboratories for the detection of circulating antiretinal antibodies have been varied and diverse, making it difficult to interpret and compare their results.
CONCLUSIONS
There is currently little standardization of laboratory methods used to detect and monitor antiretinal antibodies. To measure and monitor levels of circulating antiretinal antibodies optimally in patients with AIR, development of standardized assays with stringent internal controls is required. A multicenter collaborative and validation effort is encouraged to reach a consensus on this issue.
目的
回顾目前关于抗视网膜抗体检测和测量的文献。
设计
协作性论文。
方法
文献综述与解读。
结果
有强有力的证据表明抗视网膜抗体，尤其是那些靶向恢复蛋白和α-烯醇化酶的抗体，在自身免疫性视网膜病（AIR）的发病机制中起作用。此外，许多其他自身抗体已被描述为视网膜变性的假定介质，还有更多有待发现。然而，许多实验室在文献中描述的用于检测循环抗视网膜抗体的检测方法各不相同，难以解释和比较其结果。
结论
目前用于检测和监测抗视网膜抗体的实验室方法几乎没有标准化。为了在AIR患者中最佳地测量和监测循环抗视网膜抗体水平，需要开发具有严格内部对照的标准化检测方法。鼓励开展多中心协作和验证工作，以就该问题达成共识。
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