Corby Patricia M A, Biesbrock Aaron, Bartizek Robert, Corby Andrea L, Monteverde Robin, Ceschin Rafael, Bretz Walter A
Department of Periodontics, New York University College of Dentistry, New York, NY 10010, USA.
J Periodontol. 2008 Aug;79(8):1426-33. doi: 10.1902/jop.2008.070585.
The purpose of this study was to assess the effects of dental flossing on the microbial composition of interproximal plaque samples in matched twins.
The study was a two-treatment, examiner-masked, randomized, parallel-group, controlled study. Fifty-one twin pairs between 12 and 21 years of age were randomized to a 2-week supervised and unsupervised treatment regimen consisting of tongue brushing and toothbrushing or tongue brushing and toothbrushing plus flossing. The reverse-capture checkerboard hybridization assay was used to assess levels (abundance) of 26 microbial species in interproximal plaque samples collected from six sites per subject. An integrative computational predictive model estimated average changes in microbial abundance patterns of selected bacterial species from baseline to 2 weeks by comparing treatment groups.
After the 2-week study period, putative periodontal pathogens and cariogenic bacteria were overabundant in the group that did not floss compared to the group that performed flossing. Those included Treponema denticola, Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), Prevotella intermedia, Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), and Streptococcus mutans. Microbial species that are not consistent with the development of periodontal disease or dental caries were overabundant in the group that did floss compared to the non-flossing group.
In a well-matched twin cohort, tooth and tongue brushing plus flossing significantly decreased the abundance of microbial species associated with periodontal disease and dental caries after a 2-week program.
本研究的目的是评估牙线清洁对同卵双胞胎邻面菌斑样本微生物组成的影响。
该研究为双治疗、检查者盲法、随机、平行组对照研究。51对年龄在12至21岁之间的双胞胎被随机分配到一个为期2周的有监督和无监督治疗方案中，该方案包括舌刷和牙刷刷牙，或舌刷、牙刷刷牙加牙线清洁。采用反向捕获棋盘杂交分析法评估从每位受试者六个部位采集的邻面菌斑样本中26种微生物的水平（丰度）。一个综合计算预测模型通过比较治疗组来估计选定细菌物种从基线到2周的微生物丰度模式的平均变化。
在为期2周的研究期后，与进行牙线清洁的组相比，未进行牙线清洁的组中假定的牙周病原体和致龋菌过多。这些包括具核梭杆菌、牙龈卟啉单胞菌、福赛坦氏菌（以前称为福赛拟杆菌）、中间普氏菌、伴放线聚集杆菌（以前称为伴放线放线杆菌）和变形链球菌。与未进行牙线清洁的组相比，进行牙线清洁的组中与牙周病或龋齿发展不一致的微生物物种过多。
在一个匹配良好的双胞胎队列中，刷牙、刷舌加牙线清洁在为期2周的方案后显著降低了与牙周病和龋齿相关的微生物物种的丰度。
