Mori Koji, Suzuki Ken-ichiro
NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, Japan.
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1885-91. doi: 10.1099/ijs.0.65754-0.
A novel obligately chemolithoautotrophic, sulfur-oxidizing bacterium designated strain BDA453T was isolated from a hot spring in Fukushima prefecture, Japan. The cells were short-rod-shaped and possessed an inclusion, a Gram-negative type cell wall and a single polar flagellum. Strain BDA453T grew by sulfur-oxidizing respiration with thiosulfate, elemental sulfur, sulfide and tetrathionate as electron donors and used only carbon dioxide as a carbon source. The optimum growth conditions were 45 degrees C, pH 6.5 and the absence of NaCl. Analysis of the 16S rRNA gene revealed that the isolate was a member of the Gammaproteobacteria and related to the genera Halothiobacillus and Thiovirga in the family Halothiobacillaceae. However, the phylogenetic tree constructed using 16S rRNA gene sequences showed that strain BDA453T was distant from any other known bacteria with sequence similarities of less than 90 %. On the basis of phenotypic features and phylogenetic analysis, strain BDA453T is considered to represent a novel species of a new genus within the family Halothiobacillaceae, for which the name Thiofaba tepidiphila gen. nov., sp. nov. is proposed. The type strain of Thiofaba tepidiphila is BDA453T (=NBRC 103218T=DSM 19618T).
从日本福岛县的一处温泉中分离出了一种新型专性化能自养硫氧化细菌，命名为菌株BDA453T。细胞呈短杆状，有内含物，具革兰氏阴性型细胞壁和单根极生鞭毛。菌株BDA453T通过以硫代硫酸盐、元素硫、硫化物和连四硫酸盐作为电子供体的硫氧化呼吸进行生长，仅以二氧化碳作为碳源。最适生长条件为45℃、pH 6.5且无氯化钠。16S rRNA基因分析表明，该分离物是γ-变形菌纲的成员，与嗜盐硫杆菌科的嗜盐硫杆菌属和硫弧菌属相关。然而，使用16S rRNA基因序列构建的系统发育树显示，菌株BDA453T与任何其他已知细菌的距离较远，序列相似性低于90%。基于表型特征和系统发育分析，菌株BDA453T被认为代表嗜盐硫杆菌科内一个新属的一个新物种，为此提出新属名嗜温硫豆菌属（Thiofaba），新种名嗜温嗜硫豆菌（Thiofaba tepidiphila）。嗜温嗜硫豆菌的模式菌株为BDA453T（=NBRC 103218T=DSM 19618T）。
