Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, PR China.
Arch Microbiol. 2021 Jul;203(5):2357-2364. doi: 10.1007/s00203-021-02216-7. Epub 2021 Mar 3.
A Gram-stain-negative, aerobic, non-motile, yellow-pigmented rod-shaped and alginate-degrading bacterium, designated B1N29, was isolated from the gut of the abalone Haliotis rubra obtained in Weihai, China. Strain B1N29 was found to grow at 4-35 ℃ (optimum, 25 ℃), at pH 6.5-9.0 (optimum, 7.0-7.5) and in the presence of 0.5-9% (w/v) NaCl (optimum, 2%). Cells were positive for oxidase and catalase activity. The 16S rRNA-based phylogenetic analysis revealed that the nearest phylogenetic neighbors of strain B1N29 were Tamlana carrageenivorans KCTC 62451 (98.2%) and Tamlana agarivorans KCTC 22176 (97.7%). Based on the phylogenomic analysis, the average nucleotide identity (ANI) values between strain B1N29 and the neighbor strains were 79.2 and 79.0%, respectively; the digital DNA-DNA hybridization (dDDH) values between strain B1N29 and its two closest neighbors were 22.8 and 23.0%, respectively. Menaquinone-6 (MK-6) was detected as the sole respiratory quinone. The dominant cellular fatty acids were iso-C, iso-C 3-OH, anteiso-C and iso-C G. The polar lipids included phosphatidylethanolamine, one aminophospholipid, seven aminolipids and five unidentified lipids. Based on the phylogenetic and phenotypic characteristics, strain B1N29 is considered to represent a novel species of the genus Tamlana, for which the name Tamlana haliotis sp. nov. is proposed. The type strain is B1N29 (= KCTC 72683 = MCCC 1H00394).
一株革兰氏阴性、需氧、不运动、黄色、能降解褐藻胶的杆状细菌，被命名为 B1N29，从中国威海获得的鲍鱼 Haliotis rubra 的肠道中分离得到。B1N29 菌株在 4-35℃（最适温度 25℃）、pH6.5-9.0（最适 pH7.0-7.5）和 0.5-9%（w/v）NaCl（最适浓度 2%）条件下生长。细胞氧化酶和过氧化氢酶活性阳性。基于 16S rRNA 的系统发育分析显示，菌株 B1N29 的最近的系统发育同源菌为 Tamlana carrageenivorans KCTC 62451（98.2%）和 Tamlana agarivorans KCTC 22176（97.7%）。基于基因组分析，B1N29 菌株与相邻菌株的平均核苷酸同源性（ANI）值分别为 79.2%和 79.0%；B1N29 菌株与其两个最接近的近缘菌株的数字 DNA-DNA 杂交（dDDH）值分别为 22.8%和 23.0%。检测到menaquinone-6（MK-6）为唯一的呼吸醌。主要的细胞脂肪酸为 iso-C、iso-C 3-OH、anteiso-C 和 iso-C G。极性脂包括磷脂酰乙醇胺、一种胺磷脂、七种胺脂和五种未知脂。基于系统发育和表型特征，B1N29 菌株被认为代表了 Tamlana 属的一个新种，提议命名为 Tamlana haliotis sp. nov.。模式菌株为 B1N29（=KCTC 72683=MCCC 1H00394）。
