Takacs Peter, Gualtieri Marc, Nassiri Mehdi, Candiotti Keith, Medina Carlos A
Department of Obstetrics and Gynecology (D-50), University of Miami, Miller School of Medicine, Jackson Memorial Hospital, P.O. Box 016960, Miami, FL, 33010, USA.
Int Urogynecol J Pelvic Floor Dysfunct. 2008 Nov;19(11):1559-64. doi: 10.1007/s00192-008-0690-z. Epub 2008 Aug 2.
To compare the smooth muscle content and apoptosis of the vagina in women with and without anterior vaginal wall prolapse. Vaginal tissues were sampled in women with (n = 6) or without (n = 6) anterior vaginal wall prolapse undergoing hysterectomy. Smooth muscle of the vagina was studied by immunohistochemistry. Digital image analysis was used to determine the fractional area of smooth muscle in the histologic cross-sections. Apoptosis was assessed by TUNEL assay. The fractional area of non-vascular smooth muscle in the vagina of women with anterior vaginal wall prolapse was significantly decreased compared to women without prolapse (0.36 +/- 0.12 vs. 0.16 +/- 0.12 P = 0.021) and the apoptotic index was significantly higher compared to women without prolapse (0.04 +/- 0.01 vs. 0.02 +/- 0.03, P = 0.041). The fraction of smooth muscle in the vagina is significantly decreased and the rate of apoptosis is higher in women with anterior vaginal wall prolapse compared to women without prolapse.
比较有和无前阴道壁脱垂女性阴道的平滑肌含量及细胞凋亡情况。对接受子宫切除术的有前阴道壁脱垂（n = 6）和无前阴道壁脱垂（n = 6）的女性进行阴道组织采样。通过免疫组织化学研究阴道平滑肌。采用数字图像分析确定组织学横截面中平滑肌的部分面积。通过TUNEL检测评估细胞凋亡。与无脱垂女性相比，有前阴道壁脱垂女性阴道中非血管平滑肌的部分面积显著降低（0.36±0.12 vs. 0.16±0.12，P = 0.021），且凋亡指数显著更高（0.04±0.01 vs. 0.02±0.03，P = 0.041）。与无脱垂女性相比，有前阴道壁脱垂女性阴道中平滑肌部分显著减少，细胞凋亡率更高。
