Miller L K, Fried M
J Virol. 1976 Jun;18(3):824-32. doi: 10.1128/JVI.18.3.824-832.1976.
Seven early mutants, three late mutants, and one plaque morphology mutant of polyoma have been mapped by marker rescue using wild-type restriction endonuclease fragments. The early mutants map between 1.0 and 26.4 units from the Eco RI site, a region previously shown to correspond to the 3'-OH termainal half of "early" RNA (Kamen et al., 1974). The late mutants as well as the plaque morphology mutant map between 26.6 and 45.4 map units, a region previously shown to correspond to the 3'-OH terminal half of "late" RNA (Kamen et al., 1974). Analysis of the genotype of rescued virus demonstrated that the modification of the mutant DNA during marker rescue was limited to the region of the genome covered by the wild-type restriction endonuclease fragment tested.
利用野生型限制性内切酶片段通过标记拯救技术，已对多瘤病毒的7个早期突变体、3个晚期突变体和1个噬菌斑形态突变体进行了定位。早期突变体位于距Eco RI位点1.0至26.4个单位之间，该区域先前已显示对应于“早期”RNA的3'-OH末端一半（卡门等人，1974年）。晚期突变体以及噬菌斑形态突变体位于26.6至45.4个图谱单位之间，该区域先前已显示对应于“晚期”RNA的3'-OH末端一半（卡门等人，1974年）。对拯救病毒基因型的分析表明，标记拯救过程中突变体DNA的修饰仅限于所测试的野生型限制性内切酶片段覆盖的基因组区域。
