Spanis C W, Gutierrez M D, Drucker-Colín R R
Pharmacol Biochem Behav. 1976 Aug;5(2):165-73. doi: 10.1016/0091-3057(76)90033-2.
Twenty cats were prepared surgically with electrodes for recording the EEG, Eye Movements and EMG and a push-pull cannula system in the midbrain reticular formation (MRF) allowing the extraction of perfusates during wakefulness or REM sleep. Proteins in the perfusates were analyzed by Isoelectric Focusing (IEF) polyacrylamide gels and Sodium Dodesyl Sulphate (SDS) slab gels. In addition analysis of glycoproteins was done by gas chromatography. In some cats the contribution of cerebrospinal fluid (CSF) proteins to perfusate proteins from brain tissue was studied by intraventricular injections of labelled leucine. The effect of sleep alterations on the protein cycle during sleep and wakefulness was also studied. The results of these experiments showed that most of the proteins in the perfusates are acidic, and that REM sleep perfusates contain 2 proteins M.W. 73.000 and 45.000 not present in awake perfusates, CSF or serum. It was also shown that CSF proteins do not appear to contribute to the proteins in perfusates, and that altering the sleep wake cycle, induces changes in the rhythm of protein release in perfusates. It is suggested that some relatively large polypeptides may participate in the regulation of REM sleep.
对20只猫进行了手术准备，植入电极以记录脑电图（EEG）、眼动和肌电图（EMG），并在中脑网状结构（MRF）中植入推挽式套管系统，以便在清醒或快速眼动睡眠期间提取灌流液。通过等电聚焦（IEF）聚丙烯酰胺凝胶和十二烷基硫酸钠（SDS）平板凝胶对灌流液中的蛋白质进行分析。此外，通过气相色谱法对糖蛋白进行分析。在一些猫中，通过脑室内注射标记的亮氨酸研究脑脊液（CSF）蛋白对脑组织灌流液蛋白的贡献。还研究了睡眠改变对睡眠和清醒期间蛋白质循环的影响。这些实验结果表明，灌流液中的大多数蛋白质是酸性的，快速眼动睡眠灌流液中含有两种分子量分别为73000和45000的蛋白质，清醒灌流液、脑脊液或血清中不存在这些蛋白质。还表明脑脊液蛋白似乎对灌流液中的蛋白质没有贡献，改变睡眠-觉醒周期会导致灌流液中蛋白质释放节律的变化。有人提出，一些相对较大的多肽可能参与快速眼动睡眠的调节。
