Barna B P, Rogers L R, Thomassen M J, Barnett G H, Estes M L
Department of Immunopathology, Cleveland Clinic Foundation, OH 44195.
Cancer Immunol Immunother. 1991;33(5):314-8. doi: 10.1007/BF01756596.
Monocyte-mediated tumoricidal activity, tumor necrosis factor alpha (TNF alpha) secretion and gene expression were examined in astrocytoma patients, patients with other types of brain tumors (primary or metastatic), and normal individuals. The spontaneous monocyte-mediated tumoricidal activity of either patient group against an astrocytoma cell line was significantly greater than normal. There was no difference between patient groups. When monocytes were stimulated with lipopolysaccharide in vitro, tumoricidal activity increased in all patient groups. Patient monocyte activity tested shortly (48 h) after surgery was not different from that before surgery. Both spontaneous and stimulated monocyte cytocidal activities were tumor-cell-restricted: melanoma and astrocytoma cells were equally susceptible but non-neoplastic glial cells were not affected. Examination of monocyte TNF alpha secretion and mRNA expression indicated that patient activity was comparable to or greater than normal. These results demonstrate that, despite steroid therapy, circulating monocytes in astrocytoma and other brain tumor patients retain intact functional activity.
我们检测了星形细胞瘤患者、其他类型脑肿瘤（原发性或转移性）患者以及正常个体的单核细胞介导的杀瘤活性、肿瘤坏死因子α（TNFα）分泌及基因表达。两组患者针对星形细胞瘤细胞系的自发单核细胞介导杀瘤活性均显著高于正常个体。两组患者之间无差异。体外使用脂多糖刺激单核细胞后，所有患者组的杀瘤活性均增加。术后不久（48小时）检测的患者单核细胞活性与术前无差异。自发和刺激后的单核细胞杀细胞活性均受肿瘤细胞限制：黑色素瘤和星形细胞瘤细胞同样敏感，但非肿瘤性神经胶质细胞不受影响。对单核细胞TNFα分泌及mRNA表达的检测表明，患者的活性与正常个体相当或更高。这些结果表明，尽管接受了类固醇治疗，但星形细胞瘤和其他脑肿瘤患者的循环单核细胞仍保留完整的功能活性。
