Harada K, Yoshida J, Mizuno M, Sugita K, Kurisu K, Uozumi T
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
J Neurooncol. 1994;22(3):221-5. doi: 10.1007/BF01052922.
Using subcutaneous solid tumors produced by U251-MG human glioma cells, we studied the in vivo transfection of the cells with the tumor necrosis factor-alpha (TNF-alpha) gene delivered by means of liposomes. When the tumor had become 7 mm in diameter, liposomes with entrapped TNF-alpha gene were injected into the center of the subcutaneous tumor. We found that mRNA of transfection-induced TNF-alpha, which was expressed in the tumor tissue, was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) method and its protein was demonstrated by enzyme-linked immunoassay. Growth of the tumor was inhibited when the injection was carried out five times at every other day. The growth-inhibitory effect by transfection-induced TNF-alpha was much remarkable as compared with exogenous TNF-alpha and the effect was enhanced by the intraperitoneal injection of gamma-interferon (IFN-gamma) 12 h prior to intratumoral injection of the liposomes.
利用U251-MG人胶质瘤细胞产生的皮下实体瘤，我们研究了通过脂质体递送肿瘤坏死因子-α（TNF-α）基因对细胞进行体内转染的情况。当肿瘤直径达到7毫米时，将包裹有TNF-α基因的脂质体注射到皮下肿瘤的中心。我们发现，通过逆转录聚合酶链反应（RT-PCR）方法在肿瘤组织中检测到了转染诱导的TNF-α的mRNA，并且通过酶联免疫测定法证实了其蛋白质的存在。每隔一天进行5次注射时，肿瘤生长受到抑制。与外源性TNF-α相比，转染诱导的TNF-α的生长抑制作用更为显著，并且在瘤内注射脂质体前12小时腹腔注射γ-干扰素（IFN-γ）可增强该作用。
