Nishikawa Yoshitsugu, Yasumi Yuki, Noguchi Shunsuke, Sakamoto Hiroshi, Nikawa Jun-ichi
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan.
Biosci Biotechnol Biochem. 2008 Aug;72(8):2034-8. doi: 10.1271/bbb.80156. Epub 2008 Aug 7.
Pseudomonas putida benF, benK, benE1, and benE2 genes encode proteins belonging to benzoate transporter super family, but those functions have not yet been elucidated. In this study we analyzed the functions of these gene products using the yeast Saccharomyces cerevisiae. P. putida gene products expressed in yeast cells were localized to the yeast plasma membrane and were involved in taking up benzoate into the cells. According to the sensitivity of yeast cell-growth to benzoate, it is proposed that benK, benE1, and benE2 gene products function as transporters, that take up benzoate into the cells, whereas the benF gene product functions as an efflux pump of benzoate.
恶臭假单胞菌的benF、benK、benE1和benE2基因编码属于苯甲酸转运蛋白超家族的蛋白质，但其功能尚未阐明。在本研究中，我们利用酿酒酵母分析了这些基因产物的功能。在酵母细胞中表达的恶臭假单胞菌基因产物定位于酵母质膜，并参与将苯甲酸摄入细胞。根据酵母细胞生长对苯甲酸的敏感性，推测benK、benE1和benE2基因产物作为转运蛋白发挥作用，将苯甲酸摄入细胞，而benF基因产物则作为苯甲酸的外排泵发挥作用。
