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小球藻H⁺/己糖共转运蛋白基因。

The Chlorella H+/hexose cotransporter gene.

作者信息

Wolf K, Tanner W, Sauer N

机构信息

Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Federal Republic of Germany.

出版信息

Curr Genet. 1991 Mar;19(3):215-9. doi: 10.1007/BF00336489.

DOI:10.1007/BF00336489
PMID:1868571
Abstract

The complete genomic sequence of the inducible Chlorella kessleri H+/hexose cotransporter (HUP1) has been obtained from two overlapping clones isolated from a lambda gt10 library. The HUP1 gene is interrupted by 14 introns with the first intron being located in the 5'-untranslated part of the gene. The average intron length is 220 bp, yielding a very regular intron/exon pattern in the gene. The codon usage in this gene is strongly biased with a clear preference for C and a strong suppression of A. A consensus sequence for a putative algal polyadenylation sequence is shown and compared with other algal cDNA sequences.

摘要

已从λgt10文库中分离出的两个重叠克隆中获得了可诱导的克氏小球藻H⁺/己糖共转运蛋白（HUP1）的完整基因组序列。HUP1基因被14个内含子打断，第一个内含子位于基因的5'非翻译区。内含子的平均长度为220bp，在基因中形成了非常规则的内含子/外显子模式。该基因的密码子使用存在强烈偏向性，明显偏好C且强烈抑制A。展示了一个假定的藻类聚腺苷酸化序列的共有序列，并与其他藻类cDNA序列进行了比较。

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本文引用的文献

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Unique repetitive sequences of 170 bp inChlorella.小球藻中 170 个碱基对的独特重复序列。
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Glucose induces two amino acid transport systems in Chlorella.葡萄糖可诱导小球藻中的两种氨基酸转运系统。
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Alternative splicing of a precursor-mRNA encoded by the Chlorella sorokiniana NADP-specific glutamate dehydrogenase gene yields mRNAs for precursor proteins of isozyme subunits with different ammonium affinities.由小球藻（Chlorella sorokiniana）NADP特异性谷氨酸脱氢酶基因编码的前体mRNA的可变剪接产生了具有不同铵亲和力的同工酶亚基前体蛋白的mRNA。
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8
The Chlorella hexose/H+ symporter is a useful selectable marker and biochemical reagent when expressed in Volvox.小球藻己糖/H⁺同向转运体在团藻中表达时是一种有用的选择标记和生化试剂。
Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):669-73. doi: 10.1073/pnas.93.2.669.
9
Subcellular localization of the inducible Chlorella HUP1 monosaccharide-H+ symporter and cloning of a Co-induced galactose-H+ symporter.诱导型小球藻HUP1单糖-H⁺同向转运体的亚细胞定位及共诱导型半乳糖-H⁺同向转运体的克隆
Plant Physiol. 1995 Jan;107(1):33-41. doi: 10.1104/pp.107.1.33.
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New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
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The two beta-tubulin genes of Chlamydomonas reinhardtii code for identical proteins.莱茵衣藻的两个β-微管蛋白基因编码相同的蛋白质。
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Light-driven active uptake of 3-O-methylglucose via an inducible hexose uptake system of Chlorella.通过小球藻的诱导型己糖摄取系统实现光驱动的3-O-甲基葡萄糖主动摄取。
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Replication of chloroplast DNA in Chlamydomonas reinhardi during vegetative cell cycle: its mode and regulation.莱茵衣藻营养细胞周期中叶绿体DNA的复制：其模式与调控
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J Gen Physiol. 1974 Nov;64(5):568-81. doi: 10.1085/jgp.64.5.568.
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The two alpha-tubulin genes of Chlamydomonas reinhardi code for slightly different proteins.莱茵衣藻的两个α-微管蛋白基因编码的蛋白质略有不同。
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Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii.莱茵衣藻中编码二磷酸核酮糖羧化酶/加氧酶可变小亚基的两个基因的序列、进化及差异表达
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