Hilgarth C, Sauer N, Tanner W
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany.
J Biol Chem. 1991 Dec 15;266(35):24044-7.
A presumably full-length cDNA clone of the mitochondrial ATP/ADP translocator (AAT) of Chlorella kessleri has been isolated and sequenced. The expression of the AAT gene is highly increased in the presence of D-glucose (14 mM). At least nine more genes are activated when autotrophically grown Chlorella cells switch to heterotrophic growth. Among these is the HUP1 gene coding for the hexose transporter (Sauer, N., Caspari, T., Klebl, F., and Tanner, W. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 7949-7952) and, as also shown in this paper, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. When glucose or the nonmetabolizable analogue 6-deoxyglucose is added to the cells, an increased expression of GAPDH or AAT is observed after 10 or 30 min, respectively. Hexose uptake mutants (HUP1-) do not respond to sugars in this way, which indicates that either the inducer has to be internalized or that the HUP1 translocator is part of the signal transduction mechanism.
小球藻线粒体ATP/ADP转位酶（AAT）的一个推测为全长的cDNA克隆已被分离和测序。在D-葡萄糖（14 mM）存在的情况下，AAT基因的表达显著增加。当自养生长的小球藻细胞转变为异养生长时，至少还有九个基因被激活。其中包括编码己糖转运蛋白的HUP1基因（Sauer, N., Caspari, T., Klebl, F., and Tanner, W. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 7949 - 7952），以及如本文所示的甘油醛-3-磷酸脱氢酶（GAPDH）基因。当向细胞中添加葡萄糖或不可代谢的类似物6-脱氧葡萄糖时，分别在10分钟或30分钟后观察到GAPDH或AAT的表达增加。己糖摄取突变体（HUP1-）对糖类没有这种反应，这表明要么诱导剂必须被内化，要么HUP1转位酶是信号转导机制的一部分。
