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The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.

作者信息

Sauer N, Tanner W

机构信息

Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, FRG.

出版信息

FEBS Lett. 1989 Dec 18;259(1):43-6. doi: 10.1016/0014-5793(89)81489-9.

DOI:10.1016/0014-5793(89)81489-9
PMID:2599110
Abstract

The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to induction; it is not expressed in a hexose transport mutant. An open reading frame allows for a membrane protein of 533 amino acids with a relative molecular mass of 57 kDa. This protein is highly homologous to the human and rat glucose transporters catalyzing facilitated diffusion and to the bacterial H+/pentose cotransporters. It is not related to the H+/lactose cotransporter of E. colli and to the mammalian Na+/glucose cotransporter.

摘要

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