Dyer N A, Lawton S P, Ravel S, Choi K S, Lehane M J, Robinson A S, Okedi L M, Hall M J R, Solano P, Donnelly M J
Vector Group, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, Merseyside, L3 5QA, UK.
Mol Phylogenet Evol. 2008 Oct;49(1):227-39. doi: 10.1016/j.ympev.2008.07.011. Epub 2008 Jul 22.
Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In particular, cytochrome oxidase 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented.
基于线粒体（细胞色素氧化酶1、NADH脱氢酶2和16S）和核（核糖体DNA的内转录间隔区1）序列推断了13种舌蝇属（采采蝇）物种之间的关系。所得系统发育树证实了形态学上定义的fusc、morsitans和palpalis亚属的单系性。palpalis和morsitans亚种之间的遗传距离表明它们的分类地位需要修订。特别是，细胞色素氧化酶1序列显示在冈比亚采采蝇（Glossina palpalis palpalis）内存在较大的地理差异，表明该亚种内存在隐存种。对palpalis组雌性生殖板的形态进行了检查，发现个体在标准鉴定检索表规定的范围之外存在差异，使得无法进行明确的形态学分类。本文提出了一种基于内转录间隔区1序列长度差异来区分冈比亚采采蝇、嗜人采采蝇（G. tachinoides）和加蓬采采蝇（G. palpalis gambiensis）的诊断性PCR方法。
