泛连接蛋白是耳蜗中组装缝隙连接的新分子候选物。
Pannexins are new molecular candidates for assembling gap junctions in the cochlea.
作者信息
Tang Wenxue, Ahmad Shoeb, Shestopalov Valery I, Lin Xi
机构信息
Department of Otolaryngology, Emory University School of Medicine, Atlanta, Georgia, USA.
出版信息
Neuroreport. 2008 Aug 27;19(13):1253-7. doi: 10.1097/WNR.0b013e32830891f5.
Abstract
Genetic studies have linked many nonsyndromic deafness patients to mutations in genes coding for gap junction proteins. To better understand molecular identities of gap junctions in the cochlea, we investigated the expression of pannexins (Panxs). Western blot and reverse transcription-PCR detected the expression of Panx1 and Panx2. Immunolabeling localized Panx1 to the inner and outer sulcus, as well as the Claudius cells. Both Panx1 and Panx2 were expressed in the spiral and Scarpa's ganglion neurons. These data for the first time showed expressions of Panxs in the cochlea, therefore adding a new family of gap junction proteins to those used to form intercellular transport pathways in the cochlea.
摘要
遗传学研究已将许多非综合征性耳聋患者与编码间隙连接蛋白的基因突变联系起来。为了更好地了解耳蜗中间隙连接的分子特性，我们研究了泛连接蛋白（Panxs）的表达。蛋白质免疫印迹法和逆转录-聚合酶链反应检测到了Panx1和Panx2的表达。免疫标记将Panx1定位于内、外螺旋沟以及克劳迪乌斯细胞。Panx1和Panx2均在螺旋神经节和斯卡帕神经节神经元中表达。这些数据首次显示了泛连接蛋白在耳蜗中的表达，因此在用于形成耳蜗细胞间转运途径的间隙连接蛋白中增加了一个新的蛋白家族。
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