Kim Jeong Mi, Yun-Choi Hye Sook
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Korea.
Arch Pharm Res. 2008 Jul;31(7):886-90. doi: 10.1007/s12272-001-1242-1. Epub 2008 Aug 14.
A methanol extract of Sophora japonica was subjected to anti-platelet activity guided fractionation affording the isolation of four flavonoids and six flavonoid-glycosides: biochanin A (1), irisolidone (2), genistein (3), sissotrin (4), sophorabioside (5), genistin (6), tectoridin (7), apigenin (8), quercitrin (9), and rutin (10). The structure of each compound was determined by a variety of spectroscopic methods. Among the compounds, 1, 3, and 7 showed approximately 2.5-6.5 fold greater inhibitory effects on arachidonic acid (AA) and U46619 induced platelet aggregation (IC50: 19.9 and 99.8 microM; 20.3 and 53.8 microM; 25.9 and 123.4 microM, respectively) than acetylsalicylic acid (ASA, IC50: 63.0 and 350.0 microM). Compound 2 was an approximately 22-40 fold stronger inhibitor than ASA on AA and U46619 induced aggregation (IC50: 1.6 and 15.6 microM, respectively).
对槐米的甲醇提取物进行抗血小板活性导向分级分离，从中分离出四种黄酮类化合物和六种黄酮苷：鹰嘴豆芽素A（1）、鸢尾酮（2）、染料木黄酮（3）、异鼠李素（4）、槐二糖甙（5）、染料木苷（6）、鸢尾黄素（7）、芹菜素（8）、槲皮苷（9）和芦丁（10）。通过多种光谱方法确定了每种化合物的结构。在这些化合物中，1、3和7对花生四烯酸（AA）和U46619诱导的血小板聚集的抑制作用比阿司匹林（ASA，IC50：63.0和350.0 microM）分别高约2.5 - 6.5倍（IC50：19.9和99.8 microM；20.3和53.8 microM；25.9和123.4 microM）。化合物2对AA和U46619诱导的聚集的抑制作用比ASA强约22 - 40倍（IC50分别为1.6和15.6 microM）。
