Sapkota Dipak, Bruland Ove, Bøe Olav E, Bakeer Hala, Elgindi Osman A A, Vasstrand Endre N, Ibrahim Salah O
Department of Biomedicine, University of Bergen, Bergen, Norway.
J Oral Pathol Med. 2008 Nov;37(10):607-15. doi: 10.1111/j.1600-0714.2008.00683.x. Epub 2008 Aug 14.
Several of the S100 gene members have been reported to be differentially expressed in many human pathological conditions, in particular, the malignancies. Identification and quantification of the differentially expressed S100 gene members in oral squamous cell carcinoma (OSCC) might facilitate their use as potential diagnostic and/or prognostic markers or targets for therapy.
we examined the expression profile of 16 members of the S100 gene family at the mRNA level by semiquantitative reverse transcription-polymerase chain reaction (sRT-PCR) in 27 cases of OSCCs/their pair-wised normal controls obtained from Sudanese patients, and confirmed the sRT-PCR results by performing quantitative real time-polymerase chain reaction (qRT-PCR) for 6 of the 16 genes examined.
With sRT-PCR, 4 (25%; S100A4, S100A6, S100A8, S100A14) out of the 16 S100 gene members examined were found to be significantly down-regulated (P < 0.05) in the tumors compared to the normal controls. None of the S100 gene members examined were found to be significantly up-regulated in the tumors. qRT-PCR results confirmed the significant down-regulation of the S100A4, S100A6, and S100A14 genes in the tumors examined.
S100 gene family members might play an important role in the pathogenesis of the OSCCs examined. Findings of the present work warrant in-depth studies of the S100 gene family members, in particular, the S100A4, S100A6, S100A8, and S100A14 to further understand their possible role(s) in OSCC tumorigenesis.
据报道，几个S100基因成员在许多人类病理状况下存在差异表达，尤其是在恶性肿瘤中。鉴定和定量口腔鳞状细胞癌（OSCC）中差异表达的S100基因成员可能有助于将其用作潜在的诊断和/或预后标志物或治疗靶点。
我们通过半定量逆转录-聚合酶链反应（sRT-PCR）在mRNA水平检测了27例OSCC患者及其配对的正常对照（取自苏丹患者）中S100基因家族16个成员的表达谱，并通过对所检测的16个基因中的6个进行定量实时聚合酶链反应（qRT-PCR）来确认sRT-PCR结果。
通过sRT-PCR发现，在所检测的16个S100基因成员中，有4个（25%；S100A4、S100A6、S100A8、S100A14）在肿瘤中相对于正常对照显著下调（P < 0.05）。在所检测的S100基因成员中，未发现有在肿瘤中显著上调的。qRT-PCR结果证实了所检测肿瘤中S100A4、S100A6和S100A14基因的显著下调。
S100基因家族成员可能在所检测的OSCC发病机制中起重要作用。本研究结果值得对S100基因家族成员，尤其是S100A4、S100A6、S100A8和S100A14进行深入研究，以进一步了解它们在OSCC肿瘤发生中的可能作用。
