Kumosani T A, Elshal M F, Al-Jonaid A A, Abduljabar H S
Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box: 80203 Jeddah, 21589, Saudi Arabia.
Clin Biochem. 2008 Oct;41(14-15):1199-203. doi: 10.1016/j.clinbiochem.2008.07.013. Epub 2008 Aug 5.
Tobacco smoking is now increasing rapidly throughout the developing world and is one of the biggest threats to current and future world health. Several studies have addressed the role of cigarette smoking on semen quality, but the exact mechanisms remain inconclusive. In order to evaluate the detrimental effects of smoking on semen quality among Saudi subjects, the levels of different seminal parameters in smokers were compared to non-smokers.
A total of 159 semen samples (61 smokers and 98 non-smokers) from men attending an infertility clinic for routine infertility workup were sub-grouped into fertile or infertile and were compared based on standard semen analysis (according to WHO guidelines), content of metals (magnesium, zinc and cadmium) and plasma membrane Ca(2+)-ATPase activity of sperms.
Cadmium concentration was found significantly higher in smokers than in non-smokers either in fertile or infertile group (2.9+/-0.4 vs 1.4+/-0.7; 2.9+/-0.5 vs 1.3+/-0.7 microg L(-1); respectively). Together with this increase in seminal Cd a significant decrease in Ca(2+)-ATPase activity (21.5+/-2.8 vs 33.71+/-1.2; 20.7+/-1.5 vs 35.07+/-2.9 mmol min(-1) mg(-1) protein, p<0.05), decrease in seminal zinc (109.8+/-8.1 vs 189.7+/-9.9 mg L(-1), p<0.01) and decrease in sperm motility (41.9%+/-2.9 vs 46.01%+/-2.5; 9.8%+/-2.4 vs 15.3%+/-2.7, p<0.05) were found.
Our data demonstrate that cigarette smoking affects both Ca(2+)-ATPase activity and motility of the spermatozoa. These effects may be attributed to increased seminal cadmium and reduced zinc concentrations.
目前，吸烟在整个发展中世界正迅速增加，是对当前和未来世界健康的最大威胁之一。多项研究探讨了吸烟对精液质量的作用，但确切机制仍无定论。为了评估吸烟对沙特受试者精液质量的有害影响，将吸烟者与非吸烟者的不同精液参数水平进行了比较。
从一家不孕不育诊所接受常规不孕不育检查的男性中收集了总共159份精液样本（61名吸烟者和98名非吸烟者），根据精液是否可育进行分组，并依据标准精液分析（按照世界卫生组织指南）、金属含量（镁、锌和镉）以及精子质膜Ca(2+)-ATP酶活性进行比较。
在可育或不育组中，吸烟者的镉浓度均显著高于非吸烟者（分别为2.9±0.4对1.4±0.7；2.9±0.5对1.3±0.7微克/升）。随着精液中镉含量的增加，Ca(2+)-ATP酶活性显著降低（21.5±2.8对33.71±1.2；20.7±1.5对35.07±2.9毫摩尔/分钟/毫克蛋白，p<0.05），精液锌含量降低（109.8±8.1对189.7±9.9毫克/升，p<0.01），精子活力降低（41.9%±2.9对46.01%±2.5；9.8%±2.4对15.3%±2.7，p<0.05）。
我们的数据表明，吸烟会影响精子的Ca(2+)-ATP酶活性和活力。这些影响可能归因于精液中镉含量增加和锌浓度降低。
