Das Avizit, Rahman Mohammad Imtiazur, Ferdous Ahlan Sabah, Amin Al-, Rahman Mohammad Mahbubur, Nahar Nilufar, Uddin Md Aftab, Islam Mohammad Riazul, Khan Haseena
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Development of Fiber and Polymer Science Laboratory, BCSIR, Dhaka, Bangladesh.
PLoS One. 2017 Jun 21;12(6):e0178612. doi: 10.1371/journal.pone.0178612. eCollection 2017.
Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth, PDB). Taxol was identified and characterized by different analytical techniques (TLC, HPLC, FTIR, LC-ESI-MS/MS) following its extraction by ethyl acetate. In PDB media, this fungus was found to produce 382.2 μgL-1 of taxol which is about 7.6 x103 fold higher than the first reported endophytic fungi, Taxomyces andreanae. The extracted taxol exhibited cytotoxic activity in an in vitro culture of HeLa cancer cell line. The fungal extract also exhibited antifungal and antibacterial activities against different pathogenic strains. This is the first report of a jute endophytic fungus harboring the capacity to produce taxol and also the first reported taxol producing species that belongs to the Basidiomycota phylum, so far unknown to be a taxol producer. These findings suggest that the fungal endophyte, Grammothele lineata can be an excellent source of taxol and can also serve as a potential species for chemical and genetic engineering to enhance further the production of taxol.
条带革菌（Grammothele lineata）是我们实验室从黄麻（Corchorus olitorius acc. 2015）中分离出的一种内生真菌，被发现是一种大量生产紫杉醇的菌株。通过在简单营养培养基（马铃薯葡萄糖肉汤，PDB）中培养该真菌，提取其产生的紫杉醇及其相关化合物。用乙酸乙酯提取后，通过不同的分析技术（薄层色谱法、高效液相色谱法、傅里叶变换红外光谱法、液相色谱-电喷雾串联质谱法）对紫杉醇进行鉴定和表征。在PDB培养基中，发现这种真菌能产生382.2 μg/L的紫杉醇，这比首次报道的内生真菌安德紫杉菌（Taxomyces andreanae）高出约7.6×10³倍。提取的紫杉醇在HeLa癌细胞系的体外培养中表现出细胞毒性活性。该真菌提取物对不同病原菌也表现出抗真菌和抗菌活性。这是关于黄麻内生真菌具有产生紫杉醇能力的首次报道，也是首次报道的属于担子菌门的产紫杉醇物种，迄今为止该门未知有产紫杉醇的物种。这些发现表明，条带革菌这种内生真菌可以成为紫杉醇的优良来源，也可作为化学和基因工程的潜在物种，以进一步提高紫杉醇的产量。
