Hill W F, Jakubowski W, Akin E W, Clarke N A
Appl Environ Microbiol. 1976 Feb;31(2):254-61. doi: 10.1128/aem.31.2.254-261.1976.
The sensitivity of several microporous virus-adsorbent media for reliably detecting low levels of poliovirus from 380 and 1,900 liters of drinking water by use of the tentative standard method was investigated. The virus-adsorbent media tested were (i) nitrocellulose membrane filters, (ii) epoxy-fiber glass-asbestos filters, (iii) yarn-wound fiber glass depth filters, and (iv) epoxy-fiber glass filter tubes. Virus was adsorbed to the filter media at pH 3.5 and eluted with glycine buffer, pH 11.5. The results from 44 samples demonstrated that poliovirus was detected with a 95% reliability at mean virus input levels of 3 to 7 plaque-forming units/380 liters when 1,900 liters of water was sampled. At mean virus input levels of less than 1 to 2 plaque-forming units/380 liters, the detection reliability was 66% in 76 samples when 1,900 liters of water was sampled. No significant difference in virus detection sensitivity was observed among the various virus adsorbent media tested. Overall virus recovery efficiency ranged from 28 to 42%, with a grand average of 35%. Members of the coxsackievirus groups A and B, echovirus, and adenovirus were also detected when 380 and 1,900 liters of water were sampled. These experimental observations attest to the sensitivity of the tentative standard method for detecting low levels of virus in large volumes of drinking water.
通过使用暂行标准方法，研究了几种微孔病毒吸附介质从380升和1900升饮用水中可靠检测低水平脊髓灰质炎病毒的灵敏度。所测试的病毒吸附介质有：（i）硝酸纤维素膜过滤器；（ii）环氧玻璃-石棉过滤器；（iii）绕丝玻璃深层过滤器；（iv）环氧玻璃过滤管。病毒在pH 3.5时吸附到过滤介质上，并用pH 11.5的甘氨酸缓冲液洗脱。44个样本的结果表明，当采集1900升水样时，平均病毒输入水平为3至7个蚀斑形成单位/380升时，脊髓灰质炎病毒检测可靠性为95%。当采集1900升水样时，在76个样本中，平均病毒输入水平低于1至2个蚀斑形成单位/380升时，检测可靠性为66%。在所测试的各种病毒吸附介质之间，未观察到病毒检测灵敏度有显著差异。总体病毒回收效率在28%至42%之间，总平均为35%。当采集380升和1900升水样时，还检测到了A组和B组柯萨奇病毒、埃可病毒和腺病毒。这些实验观察结果证明了暂行标准方法在检测大量饮用水中低水平病毒方面的灵敏度。
