Bhagwat Arvind A, Bhagwat Medha
Produce Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705-2350, USA.
Foodborne Pathog Dis. 2008 Aug;5(4):487-97. doi: 10.1089/fpd.2008.0117.
A comparison of genome sequences and of encoded proteins with the database of existing annotated sequences is a useful approach to understand the information at the genome level. Here we demonstrate the utility of several DNA and protein sequence comparison tools to interpret the information obtained from several genome projects. Comparisons are presented between closely related strains of Escherichia coli commensal isolates, different isolates of O157:H7, and Shigella spp. It is expected that comparative genome analysis will generate a wealth of data to compare pathogenic isolates with varying levels of pathogenicity, which in turn may reveal mechanisms by which the pathogen may adapt to a particular nutrient supply in certain foods. These genome sequence analysis tools will strengthen foodborne pathogen surveillance and subsequent risk assessment to enhance the safety of the food supply.
将基因组序列和编码蛋白质与现有注释序列数据库进行比较，是在基因组水平上理解信息的一种有用方法。在这里，我们展示了几种DNA和蛋白质序列比较工具在解释从多个基因组计划中获得的信息方面的效用。文中呈现了大肠杆菌共生分离株的密切相关菌株、不同的O157:H7分离株和志贺氏菌属之间的比较。预计比较基因组分析将产生大量数据，以比较具有不同致病水平的致病分离株，这反过来可能揭示病原体适应某些食物中特定营养供应的机制。这些基因组序列分析工具将加强食源性病原体监测及后续风险评估，以提高食品供应的安全性。
