通过免疫扩散法检测传染性胃肠炎病毒抗体。
The detection of transmissible gastroenteritis viral antibodies by immunodiffusion.
作者信息
Bohac J, Derbyshire J B
出版信息
Can J Comp Med. 1976 Apr;40(2):161-5.
Precipitating antibodies against transmissible gastroenteritis viral antigens were detected by the immunodiffusion test in two transmissible gastroenteritis viral hyperimmune antisera and in antiserum prepared against haemagglutinating encephalomyelitis virus but not in sera from several species of normal animals, in antisera prepared against a variety of othet viruses and bacteria or sera from swine with bacterial enteritis. When the immunodiffusion test was compared with the virus neutralization test for the detection of transmissible gastroeneritis viral antibodies in 20 swine sera certain samples which contained high titres of virus neutralizing antibodies failed to produce precipitation while other sera were positive in the immunodiffusion test although their virus neutralizing antibody titres were relatively low. Precipitating antibodies were also detected by immunodiffusion in several samples of milk whey from a sow which had been vaccinated with inactivated transmissible gastroenteritis virus.
通过免疫扩散试验在两种传染性胃肠炎病毒超免疫抗血清以及针对血凝性脑脊髓炎病毒制备的抗血清中检测到了针对传染性胃肠炎病毒抗原的沉淀抗体，但在几种正常动物的血清中、针对多种其他病毒和细菌制备的抗血清或患有细菌性肠炎的猪的血清中未检测到。当将免疫扩散试验与病毒中和试验用于检测20份猪血清中的传染性胃肠炎病毒抗体时，某些含有高滴度病毒中和抗体的样本未能产生沉淀，而其他血清在免疫扩散试验中呈阳性，尽管其病毒中和抗体滴度相对较低。通过免疫扩散在一头接种了灭活传染性胃肠炎病毒的母猪的几份乳清样本中也检测到了沉淀抗体。
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