Thorsen J, Djurickovic S
Can J Comp Med. 1971 Apr;35(2):99-102.
Four sows were immunized with inactivated TGE virus. The virus was either propagated in pig kidney cell cultures, or obtained from the intestines of experimentally infected gnotobiotic pigs, and inactivated by treatment with beta-propiolactone. The inactivated virus preparations were administered to sows by intramammary inoculation. Two sows received two inoculations; the other two sows received three inoculations of inactivated virus. The antibody responses in the serum and milk were determined and piglets nursing the sows were experimentally challenged with virulent TGE virus. Three inoculations of the virus preparation stimulated much higher levels of serum and milk antibody than did two inoculations. A schedule of three inoculations of sows with inactivated TGE virus was effective in stimulating protection against TGE for piglets nursing these sows.
用灭活的传染性胃肠炎（TGE）病毒对4头母猪进行免疫。该病毒要么在猪肾细胞培养物中繁殖，要么从实验感染的无菌猪的肠道中获取，并用β-丙内酯处理使其灭活。通过乳房内接种将灭活的病毒制剂施用于母猪。2头母猪接受两次接种；另外2头母猪接受三次灭活病毒接种。测定血清和乳汁中的抗体反应，并用强毒TGE病毒对吃这些母猪奶的仔猪进行实验性攻毒。与两次接种相比，三次接种病毒制剂刺激产生的血清和乳汁抗体水平要高得多。用灭活的TGE病毒对母猪进行三次接种的方案能有效刺激对吃这些母猪奶的仔猪的TGE保护作用。
