Lyon J A, Hinshaw V S
Department of Molecular Biology, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
J Gen Virol. 1991 Aug;72 ( Pt 8):2011-3. doi: 10.1099/0022-1317-72-8-2011.
The virulent avian influenza virus A/Ty/Ont/7732/66 (H5N9) (Ty/Ont) causes severe destruction of the lymphoid cells in infected birds. Previous studies have suggested that viral infection of macrophages may be involved. However, Ty/Ont failed to replicate productively in primary cultures of chicken macrophages. Therefore, in an effort to develop an in vitro system for our studies, we examined the susceptibility of an avian macrophage cell line, HD11, to Ty/Ont. We found that Ty/Ont replicated in the HD11 cells to high titres, as measured by haemagglutination (HA) assays and infectivity yields. To determine whether this property was unique to Ty/Ont, we also examined the replication of influenza viruses representative of all 13 HA subtypes and an attenuated variant of Ty/Ont. All of the tested viruses replicated in HD11 cells; the avirulent strains required the presence of trypsin in the culture medium whereas virulent viruses and the attenuated variant of Ty/Ont did not. These results suggest that the HD11 cells can support the replication of a wide variety of influenza viruses and that this continuous avian cell line may prove useful for in vitro studies on these viruses.
强毒禽流感病毒A/Ty/Ont/7732/66（H5N9）（Ty/Ont）可导致受感染禽类的淋巴细胞严重受损。先前的研究表明，巨噬细胞的病毒感染可能与之有关。然而，Ty/Ont无法在鸡巨噬细胞原代培养物中有效复制。因此，为了开发用于我们研究的体外系统，我们检测了禽类巨噬细胞系HD11对Ty/Ont的易感性。我们发现，通过血凝（HA）试验和感染性产量测定，Ty/Ont可在HD11细胞中高滴度复制。为了确定此特性是否为Ty/Ont所特有，我们还检测了代表所有13种HA亚型的流感病毒以及Ty/Ont的减毒株的复制情况。所有测试病毒均可在HD11细胞中复制；无毒株在培养基中需要胰蛋白酶，而强毒株和Ty/Ont的减毒株则不需要。这些结果表明，HD11细胞可支持多种流感病毒的复制，并且这种连续的禽类细胞系可能对这些病毒的体外研究有用。
