Li X, Swaggerty C L, Kogut M H, Chiang H, Wang Y, Genovese K J, He H, Stern N J, Pevzner I Y, Zhou H
Department of Poultry Science, Texas A&M University, College Station 77843, USA.
Poult Sci. 2008 Sep;87(9):1742-7. doi: 10.3382/ps.2008-00136.
Campylobacter jejuni is one of the most common causes of acute enteritis worldwide. Chickens are believed to be the main reservoir of C. jejuni. The role that host genetics play in resistance/susceptibility to C. jejuni colonization in broilers is still not clear. Day-old broilers from 2 parental lines (A and B) and their F(1) reciprocal crosses (C and D) were challenged orally with 10(5) cfu of C. jejuni to address the role of genetics in determining resistance/susceptibility to C. jejuni colonization in broilers. Cloacal swabs were collected on 6, 10, and 13 d postinoculation (dpi), and cecal contents cultured for C. jejuni on 7 and 14 dpi. The number of C. jejuni colonies in the cloacal swabs and cecal contents of each bird were recorded at each time point. Significantly fewer bacteria were found in the cecal contents from line A than B (P < 0.05) and cross D (A male x B female) when compared with cross C (A female x B male) at both 7 and 14 dpi. There was a significant correlation between C. jejuni counts in cloacal swabs and those in cecal contents. The results indicated that a paternal effect might be one of the important genetic factors influencing resistance to C. jejuni colonization in broilers.
空肠弯曲菌是全球急性肠炎最常见的病因之一。鸡被认为是空肠弯曲菌的主要宿主。宿主遗传学在肉鸡对空肠弯曲菌定植的抗性/易感性中所起的作用仍不清楚。选用来自2个亲本系（A和B）的一日龄肉鸡及其F(1)正反交组合（C和D），口服接种10(5) cfu空肠弯曲菌，以研究遗传学在决定肉鸡对空肠弯曲菌定植的抗性/易感性中的作用。在接种后6、10和13天（dpi）采集泄殖腔拭子，并在接种后7和14天对盲肠内容物进行空肠弯曲菌培养。记录每个时间点每只鸡泄殖腔拭子和盲肠内容物中空肠弯曲菌菌落的数量。在7和14 dpi时，A系肉鸡盲肠内容物中的细菌数量显著少于B系（P < 0.05），且与正反交组合C（A♀×B♂）相比，正反交组合D（A♂×B♀）中的细菌数量也显著更少。泄殖腔拭子和盲肠内容物中空肠弯曲菌数量之间存在显著相关性。结果表明，父本效应可能是影响肉鸡对空肠弯曲菌定植抗性的重要遗传因素之一。
