McCreary C, Martelli F, Grierson S, Ostanello F, Nevel A, Banks M
Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire AL9 7TA.
Vet Rec. 2008 Aug 30;163(9):261-5. doi: 10.1136/vr.163.9.261.
Five faecal samples were collected from four different stages of production at each of 10 pig farms in the Yorkshire Humberside area of the UK, and samples of slurry were collected from nine of the farms. All the samples were tested for hepatitis E virus (HEV) RNA by a nested reverse transcriptase PCR. At least one sample from the pigs on each of the farms tested positive for hev; its prevalence in the 10 herds varied from 5 per cent to 35 per cent and its mean prevalence was 21.5 per cent. The mean prevalence in pigs aged three to five weeks was 26.0 per cent, in pigs aged 10 to 12 weeks 44.0 per cent, in pigs aged 22 to 24 weeks 8.9 per cent, and in adult dry sows 6.0 per cent. Two of the nine slurry lagoons tested positive for HEV RNA. Phylogenetic analysis of the sequence data indicated that the strains of the virus were of genotype 3 and closely related to strains detected in other pigs and in human beings in the UK.
在英国约克郡亨伯赛德地区的10个养猪场中，每个猪场在生产的四个不同阶段采集了5份粪便样本，并从其中9个猪场采集了粪肥样本。所有样本均通过巢式逆转录聚合酶链反应检测戊型肝炎病毒（HEV）RNA。每个猪场的猪至少有一份样本检测出戊型肝炎病毒呈阳性；其在10个猪群中的流行率从5%到35%不等，平均流行率为21.5%。3至5周龄猪的平均流行率为26.0%，10至12周龄猪为44.0%，22至24周龄猪为8.9%，成年空怀母猪为6.0%。9个粪肥池中有2个检测出戊型肝炎病毒RNA呈阳性。序列数据的系统发育分析表明，该病毒株为3型，与在英国其他猪和人类中检测到的毒株密切相关。
