Banks M, Heath G S, Grierson S S, King D P, Gresham A, Girones R, Widen F, Harrison T J
Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB.
Vet Rec. 2004 Feb 21;154(8):223-7. doi: 10.1136/vr.154.8.223.
Samples of serum, tissue and faeces from two pig herds in England were examined for hepatitis E virus by reverse-transcriptase PCR (RT-PCR), and a virus strain from each herd was partially sequenced. Eleven of 42 faecal samples and 16 of 21 tissue samples from two pigs were positive for the virus by RT-PCR. Analysis of two unique but closely related nucleotide sequences obtained from the two herds showed that the viruses clustered in genotype III (6) with a human strain of the virus from an autochthonously acquired case of acute hepatitis in the UK. An ELISA based on recombinant open reading frame 2 (ORF-2) was used to detect antibodies to hepatitis E virus in 256 pig sera from the UK; 85.5 per cent of the samples were positive, compared with 58 per cent of similar samples from Swedish pigs and 23.5 per cent of samples from Dutch pigs.
通过逆转录聚合酶链反应（RT-PCR）对来自英国两个猪群的血清、组织和粪便样本进行了戊型肝炎病毒检测，并对每个猪群的一株病毒进行了部分测序。42份粪便样本中的11份以及来自两头猪的21份组织样本中的16份通过RT-PCR检测出该病毒呈阳性。对从两个猪群获得的两个独特但密切相关的核苷酸序列进行分析表明，这些病毒聚集在基因型III（6）中，与英国一例本地获得的急性肝炎患者的人源病毒株相同。使用基于重组开放阅读框2（ORF-2）的酶联免疫吸附测定（ELISA）检测了来自英国的256份猪血清中的戊型肝炎病毒抗体；85.5%的样本呈阳性，相比之下，来自瑞典猪的类似样本中有58%呈阳性，来自荷兰猪的样本中有23.5%呈阳性。
