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秀丽隐杆线虫生殖系中的基因转换和末端连接修复双链断裂。

Gene conversion and end-joining-repair double-strand breaks in the Caenorhabditis elegans germline.

作者信息

Robert Valérie J, Davis M Wayne, Jorgensen Erik M, Bessereau Jean-Louis

机构信息

Ecole Normale Supérieure, Biologie Cellulaire de la Synapse, Paris, France.

出版信息

Genetics. 2008 Sep;180(1):673-9. doi: 10.1534/genetics.108.089698. Epub 2008 Aug 30.

DOI:10.1534/genetics.108.089698
PMID:18757928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2535717/
Abstract

Excision of a Mos1 transposon in the germline of Caenorhabditis elegans generates a double-strand break in the chromosome. We demonstrate that breaks are most prominently repaired by gene conversion from the homolog, but also rarely by nonhomologous end-joining. In some cases, gene conversion events are resolved by crossing over. Surprisingly, expression of the transposase using an intestine-specific promoter can induce repair, raising the possibility that activation of transposase expression in somatic cells can lead to transposition of Mos1 in the germline.

摘要

切除秀丽隐杆线虫生殖系中的Mos1转座子会在染色体上产生双链断裂。我们证明，断裂最主要通过同源基因转换进行修复，但也很少通过非同源末端连接进行修复。在某些情况下，基因转换事件通过交叉得以解决。令人惊讶的是，使用肠道特异性启动子表达转座酶可诱导修复，这增加了体细胞中转座酶表达激活可能导致生殖系中Mos1转座的可能性。

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Environmental RNA interference.环境RNA干扰
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