Benković Vesna, Kopjar Nevenka, Horvat Knezevic Anica, Dikić Domagoj, Basić Ivan, Ramić Snjezana, Viculin Tomislav, Knezević Fabijan, Orolić Nada
Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Biol Pharm Bull. 2008 Sep;31(9):1778-85. doi: 10.1248/bpb.31.1778.
This in vitro study aimed at investigating the possible radioprotective effects of natural substances propolis and quercetin on gamma-irradiated human white blood cells. The levels of primary DNA damage were studied by the alkaline comet assay, while the cytogenetic damage was evaluated using the analysis of structural chromosome aberration and cytokinesis-block micronucleus assay. The results obtained by all endpoints indicate acceptable toxicity profiles of propolis and quercetin in vitro, and also confirmed their radioprotective abilities. Propolis was found to be more effective in diminishing the levels of primary and more complex cytogenetic DNA damage in gamma-irradiated white blood cells. Data gathered in present study support the use of propolis and quercetin as non-toxic protective substances. However, to clarify the underlying mechanisms of their cyto/radioprotective activities, additional studies are necessary at both in vitro and in vivo levels.
这项体外研究旨在探究天然物质蜂胶和槲皮素对γ射线辐照的人类白细胞可能具有的辐射防护作用。通过碱性彗星试验研究原发性DNA损伤水平，同时利用染色体结构畸变分析和胞质分裂阻断微核试验评估细胞遗传损伤。所有终点指标获得的结果表明，蜂胶和槲皮素在体外具有可接受的毒性特征，同时也证实了它们的辐射防护能力。研究发现，蜂胶在降低γ射线辐照白细胞中原发性和更复杂的细胞遗传DNA损伤水平方面更有效。本研究收集的数据支持将蜂胶和槲皮素用作无毒保护物质。然而，为了阐明其细胞/辐射防护活性的潜在机制，有必要在体外和体内水平进行更多研究。
