Benkovic Vesna, Knezevic Anica Horvat, Orsolic Nada, Basic Ivan, Ramic Snjezana, Viculin Tomislav, Knezevic Fabijan, Kopjar Nevenka
Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, Croatia.
Phytother Res. 2009 Aug;23(8):1159-68. doi: 10.1002/ptr.2774.
This in vitro study aimed to evaluate the possible radioprotective effects of the natural substances WSDP, caffeic acid, chrysin and naringin on gamma-irradiated human white blood cells. The effectiveness of tested compounds was evaluated using the alkaline comet assay, the analysis of structural chromosome aberration and the cytokinesis-block micronucleus assay. The results obtained by the alkaline comet study indicate favourable toxicity profiles of propolis and its polyphenolic components, and confirmed the radioprotective abilities comparable to the chemical radioprotector AET. WSDP and its polyphenolic components were able to reduce the number of necrotic cells. None of tested compounds induced significant genotoxicity, but all of them offered a quite measurable protection against DNA damage. WSDP was found to be the most effective in diminishing the levels of primary and more complex cytogenetic DNA damage in white blood cells. Considering its complex composition, to undoubtedly explain the underlying mechanisms of cyto/radioprotective effects, further studies are needed.
这项体外研究旨在评估天然物质WSDP、咖啡酸、白杨素和柚皮苷对γ射线辐照的人类白细胞可能的辐射防护作用。使用碱性彗星试验、染色体结构畸变分析和胞质分裂阻滞微核试验评估受试化合物的有效性。碱性彗星试验获得的结果表明蜂胶及其多酚成分具有良好的毒性特征，并证实其辐射防护能力与化学辐射防护剂AET相当。WSDP及其多酚成分能够减少坏死细胞的数量。受试化合物均未诱导明显的遗传毒性，但它们都对DNA损伤提供了相当可观的保护。发现WSDP在减少白细胞中初级和更复杂的细胞遗传学DNA损伤水平方面最为有效。考虑到其复杂的成分，无疑需要进一步研究来解释细胞/辐射防护作用的潜在机制。
