Benković Vesna, Orsolić Nada, Knezević Anica Horvat, Ramić Snjezana, Dikić Domagoj, Basić Ivan, Kopjar Nevenka
Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb,Croatia.
Biol Pharm Bull. 2008 Jan;31(1):167-72. doi: 10.1248/bpb.31.167.
The radioprotective effects of water-soluble derivate of propolis (WSDP) collected in Croatia, and single flavonoids, caffeic acid, chrysin and naringin in the whole-body irradiated CBA mice were investigated. Irradiation was performed using a gamma-ray source ((60)Co), and absorbed doses were 4 and 9 Gy. The efficiency of test components was evaluated when given intraperitoneally (i.p.) at dose of 100 mg kg(-1) for 3 consecutive days before and/or after irradiation. Moreover, possible genotoxic effects of all test components were assessed on non-irradiated animals. The higher efficiency of test components was observed when given preventively. The results suggest that propolis and related flavonoids given to mice before irradiation protected mice from lethal effects of whole-body irradiation and diminish primary DNA damage in their white blood cells as detected by the alkaline comet assay.
研究了在克罗地亚采集的水溶性蜂胶衍生物（WSDP）以及单一黄酮类化合物咖啡酸、白杨素和柚皮苷对全身照射的CBA小鼠的辐射防护作用。使用γ射线源（⁶⁰Co）进行照射，吸收剂量为4 Gy和9 Gy。在照射前和/或照射后连续3天腹腔注射（i.p.）100 mg kg⁻¹剂量的测试成分，评估其效果。此外，还评估了所有测试成分对未受照射动物可能的遗传毒性作用。预防性给药时观察到测试成分的效率更高。结果表明，照射前给小鼠服用蜂胶及相关黄酮类化合物可保护小鼠免受全身照射的致死效应，并减少碱性彗星试验检测到的其白细胞中的原发性DNA损伤。
