Oliveira Serafim M, Mijares Dindo Q, Turner Gloria, Amaral Isabel F, Barbosa Mário A, Teixeira Cristina C
Department of Mechanical Engineering, ESTV-Escola Superior de Tecnologia de Viseu, Viseu, Portugal.
Tissue Eng Part A. 2009 Mar;15(3):635-43. doi: 10.1089/ten.tea.2008.0052.
The use of biomaterials to replace lost bone has been a common practice for decades. More recently, the demands for bone repair and regeneration have pushed research into the use of cultured cells and growth factors in association with these materials. Here we report a novel approach to engineer new bone using a transient cartilage scaffold to induce endochondral ossification. Chondrocyte/chitosan scaffolds (both a transient cartilage scaffold-experimental-and a permanent cartilage scaffold-control) were prepared and implanted subcutaneously in nude mice. Bone formation was evaluated over a period of 5 months. Mineralization was assessed by Faxitron, micro computed tomography, backscatter electrons, and Fourier transform infrared spectroscopy analyses. Histological analysis provided further information on tissue changes in and around the implanted scaffolds. The deposition of ectopic bone was detected in the surface of the experimental implants as early as 1 month after implantation. After 3 months, bone trabeculae and bone marrow cavities were formed inside the scaffolds. The bone deposited was similar to the bone of the mice vertebra. Interestingly, no bone formation was observed in control implants. In conclusion, an engineered transient cartilage template carries all the signals necessary to induce endochondral bone formation in vivo.
几十年来，使用生物材料替代缺失的骨骼一直是一种常见的做法。最近，对骨修复和再生的需求推动了关于将培养细胞和生长因子与这些材料联合使用的研究。在此，我们报告一种利用临时软骨支架诱导软骨内成骨来构建新骨的新方法。制备了软骨细胞/壳聚糖支架（一种临时软骨支架——实验组——和一种永久性软骨支架——对照组）并皮下植入裸鼠体内。在5个月的时间里评估骨形成情况。通过Faxitron、微型计算机断层扫描、背散射电子和傅里叶变换红外光谱分析评估矿化情况。组织学分析提供了关于植入支架内部及周围组织变化的更多信息。早在植入后1个月，就在实验组植入物表面检测到异位骨的沉积。3个月后，支架内部形成了骨小梁和骨髓腔。沉积的骨与小鼠椎骨的骨相似。有趣的是，在对照组植入物中未观察到骨形成。总之，一种构建的临时软骨模板携带了在体内诱导软骨内骨形成所需的所有信号。
