Cansev Mehmet, Ulus Ismail H, Wang Lei, Maher Timothy J, Wurtman Richard J
Massachusetts Institute of Technology, Department of Brain and Cognitive Sciences, Cambridge, MA 02139, USA.
Neurosci Res. 2008 Nov;62(3):206-9. doi: 10.1016/j.neures.2008.07.005. Epub 2008 Aug 3.
Administering uridine-5'-monophosphate (UMP) and docosahexaenoic acid (DHA) increases synaptic membranes (as characterized by pre- and post-synaptic proteins) and dendritic spines in rodents. We examined their effects on rotational behavior and dopaminergic markers in rats with partial unilateral 6-hydroxydopamine (6-OHDA)-induced striatal lesions. Rats receiving UMP, DHA, both, or neither, daily, and intrastriatal 6-OHDA 3 days after treatment onset, were tested for d-amphetamine-induced rotational behavior and dopaminergic markers after 24 and 28 days, respectively. UMP/DHA treatment reduced ipsilateral rotations by 57% and significantly elevated striatal dopamine, tyrosine hydroxylase (TH) activity, TH protein and synapsin-1 on the lesioned side. Hence, giving uridine and DHA may partially restore dopaminergic neurotransmission in this model of Parkinson's disease.
给予尿苷-5'-单磷酸（UMP）和二十二碳六烯酸（DHA）可增加啮齿动物的突触膜（以前突触和后突触蛋白为特征）和树突棘。我们研究了它们对部分单侧6-羟基多巴胺（6-OHDA）诱导的纹状体损伤大鼠旋转行为和多巴胺能标记物的影响。在治疗开始3天后接受每日UMP、DHA、两者或两者都不给予，以及纹状体内注射6-OHDA的大鼠，分别在24天和28天后测试其对d-苯丙胺诱导的旋转行为和多巴胺能标记物的影响。UMP/DHA治疗使同侧旋转减少了57%，并显著提高了损伤侧纹状体多巴胺、酪氨酸羟化酶（TH）活性、TH蛋白和突触素-1。因此，给予尿苷和DHA可能在帕金森病的这个模型中部分恢复多巴胺能神经传递。
