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使用微立体光刻技术和表面改性开发3D PPF/DEF支架。

Development of 3D PPF/DEF scaffolds using micro-stereolithography and surface modification.

作者信息

Lan Phung Xuan, Lee Jin Woo, Seol Young-Joon, Cho Dong-Woo

机构信息

Department of Mechanical Engineering, Hanoi University of Technology, Hanoi City, Vietnam.

出版信息

J Mater Sci Mater Med. 2009 Jan;20(1):271-9. doi: 10.1007/s10856-008-3567-2. Epub 2008 Sep 3.

Abstract

Poly(propylene fumarate) (PPF) is an ultraviolet-curable and biodegradable polymer with potential applications for bone regeneration. In this study, we designed and fabricated three-dimensional (3D) porous scaffolds based on a PPF polymer network using micro-stereolithography (MSTL). The 3D scaffold was well fabricated with a highly interconnected porous structure and porosity of 65%. These results provide a new scaffold fabrication method for tissue engineering. Surface modification is a commonly used and effective method for improving the surface characteristics of biomaterials without altering their bulk properties that avoids the expense and long time associated with the development of new biomaterials. Therefore, we examined surface modification of 3D scaffolds by applying accelerated biomimetic apatite and arginine-glycine-aspartic acid (RGD) peptide coating to promote cell behavior. The apatite coating uniformly covered the scaffold surface after immersion for 24 h in 5-fold simulated body fluid (5SBF) and then the RGD peptide was applied. Finally, the coated 3D scaffolds were seeded with MC3T3-E1 pre-osteoblasts and their biologic properties were evaluated using an MTS assay and histologic staining. We found that 3D PPF/diethyl fumarate (DEF) scaffolds fabricated with MSTL and biomimetic apatite coating can be potentially used in bone tissue engineering.

摘要

聚富马酸丙二醇酯(PPF)是一种可紫外线固化且可生物降解的聚合物,在骨再生方面具有潜在应用价值。在本研究中,我们使用微立体光刻技术(MSTL)基于PPF聚合物网络设计并制造了三维(3D)多孔支架。该3D支架制造良好,具有高度互连的多孔结构,孔隙率为65%。这些结果为组织工程提供了一种新的支架制造方法。表面改性是一种常用且有效的方法,用于改善生物材料的表面特性而不改变其整体性质,避免了开发新生物材料所涉及的费用和长时间问题。因此,我们通过应用加速仿生磷灰石和精氨酸 - 甘氨酸 - 天冬氨酸(RGD)肽涂层来促进细胞行为,研究了3D支架的表面改性。在5倍模拟体液(5SBF)中浸泡24小时后,磷灰石涂层均匀覆盖支架表面,然后应用RGD肽。最后,将涂覆的3D支架接种MC3T3 - E1前成骨细胞,并使用MTS测定法和组织学染色评估其生物学特性。我们发现,用MSTL和仿生磷灰石涂层制造的3D PPF/富马酸二乙酯(DEF)支架可能潜在地用于骨组织工程。

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