Hu Lingfei, Pan Hailian, Xue Yanfen, Ventosa A, Cowan D A, Jones B E, Grant W D, Ma Yanhe
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1705-8. doi: 10.1099/ijs.0.65700-0.
A novel halophilic archaeon, strain CGSA15(T), was isolated from water of Lake Chagannor in China. The strain grew optimally at 33-37 degrees C, pH 9.5-10.0 and 4.0-4.3 M NaCl. The major polar lipids were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. The genomic DNA G+C content of strain CGSA15(T) was 60.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CGSA15(T) was a member of the genus Halorubrum and was related most closely to Halorubrum alkaliphilum AS 1.3528(T) (96.1 % similarity) and Halorubrum tibetense AS 1.3239(T) (96.9 %). Levels of DNA-DNA relatedness between strain CGSA15(T) and Hrr. alkaliphilum AS 1.3528(T) and Hrr. tibetense AS 1.3239(T) were 36.7 and 28.9 %, respectively. According to the phenotypic and genotypic data presented, strain CGSA15(T) is considered to represent a novel species of the genus Halorubrum, for which the name Halorubrum luteum sp. nov. is proposed. The type strain is CGSA15(T) (=CGMCC 1.6783(T) =CECT 7303(T)).
从中国查干诺尔湖的水中分离出了一种新型嗜盐古菌，菌株CGSA15(T)。该菌株在33 - 37℃、pH 9.5 - 10.0和4.0 - 4.3 M NaCl条件下生长最佳。主要极性脂为磷脂酰甘油和磷脂酰甘油磷酸甲酯。菌株CGSA15(T)的基因组DNA G + C含量为60.2 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株CGSA15(T)是嗜盐红菌属的成员，与嗜碱嗜盐红菌AS 1.3528(T)（相似度96.1%）和西藏嗜盐红菌AS 1.3239(T)（相似度96.9%）关系最为密切。菌株CGSA15(T)与嗜碱嗜盐红菌AS 1.3528(T)以及西藏嗜盐红菌AS 1.3239(T)之间的DNA - DNA相关性水平分别为36.7%和28.9%。根据所提供的表型和基因型数据，菌株CGSA15(T)被认为代表嗜盐红菌属的一个新物种，为此提出新名称嗜盐黄红菌（Halorubrum luteum sp. nov.）。模式菌株为CGSA15(T)（=CGMCC 1.6783(T) =CECT 7303(T)）。
