Tejesvi Mysore V, Kini Kukkundoor R, Prakash Harishchandra S, Subbiah Ven, Shetty Hunthrike S
Department of Studies in Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore-570 006, Karnataka, India.
Can J Microbiol. 2008 Sep;54(9):769-80. doi: 10.1139/w08-070.
Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The antioxidant and antihypertensive properties of Pestalotiopsis isolates were determined by measuring 1,1-diphenyl-2-picrylhydrazyl inhibitory activity, lipid peroxidation, and angiotensin-converting enzyme inhibition activity. Pestalotiopsis isolates of T. arjuna origin exhibited maximum radical scavenging activity compared with the others. The IC50 values of Pestalotiopsis extracts for 1,1-diphenyl-2-picrylhydrazyl scavenging activity ranged from 14 to 27 microg/mL compared with 15 and 6 microg/mL for butylated hydroxytoluene and ascorbic acid, respectively. The DNA damage study was also done for three isolates, TC-315, TA-37, and TA-60; TA-37 gave 80% protection. The IC50 values of Pestalotiopsis extracts for lipid peroxidation ranged between 30 and 35.5 microg/mL, while for the positive control butylated hydroxytoluene, it was 26 microg/mL. Out of 32 fungal extracts screened for antihypertensive assay, five (TA-37, TA-60, TA-102, TA-103, and TC-320) showed >60% inhibition of angiotensin-converting enzyme. The IC50 values for five extracts ranged from 21 to 37 microg/mL and was 20 microg/mL for captopril used as a positive control. The antibacterial activity was measured by the microplate-based turbidity measurement method. Four Pestalotiopsis extracts (TA-04, TA-37, TA-60, and TA-102) showed >75% inhibition against five bacterial strains including Bacillus subtilis, Escherichia coli, Pseudomonas fluorescens, Xanthomonas axonopodis pv. malvacearum, and Staphylococcus aureus. The antioxidant, antibacterial, and antihypertensive activities demonstrated the potential of Pestalotiopsis extracts as therapeutic targets.
拟盘多毛孢属物种是从四味药用植物中分离出的最主要内生真菌物种，这四味药用植物分别是：印度楝、诃子、印楝和止泻木。从这些药用植物不同部位分离出的30种拟盘多毛孢属物种被选作研究对象。通过测定1,1 - 二苯基 - 2 - 苦基肼自由基清除活性、脂质过氧化和血管紧张素转换酶抑制活性，来确定拟盘多毛孢属分离物的抗氧化和抗高血压特性。与其他分离物相比，源自印度楝的拟盘多毛孢属分离物表现出最大的自由基清除活性。拟盘多毛孢属提取物对1,1 - 二苯基 - 2 - 苦基肼自由基清除活性的IC50值在14至27微克/毫升之间，而丁基化羟基甲苯和抗坏血酸的该值分别为15和6微克/毫升。还对三种分离物TC - 315、TA - 37和TA - 60进行了DNA损伤研究；TA - 37提供了80%的保护。拟盘多毛孢属提取物对脂质过氧化的IC50值在30至35.5微克/毫升之间，而阳性对照丁基化羟基甲苯的该值为26微克/毫升。在筛选用于抗高血压测定的32种真菌提取物中，有五种（TA - 37、TA - 60、TA - 102、TA - 103和TC - 320）对血管紧张素转换酶的抑制率>60%。这五种提取物的IC50值在21至37微克/毫升之间，用作阳性对照的卡托普利的该值为20微克/毫升。通过基于微孔板的比浊法测量抗菌活性。四种拟盘多毛孢属提取物（TA - 04、TA - 37、TA - 60和TA - 102）对包括枯草芽孢杆菌、大肠杆菌、荧光假单胞菌、锦葵黄单胞菌和金黄色葡萄球菌在内的五种细菌菌株的抑制率>75%。抗氧化、抗菌和抗高血压活性表明拟盘多毛孢属提取物具有作为治疗靶点的潜力。
