Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy.
J Appl Microbiol. 2008 Nov;105(5):1266-76. doi: 10.1111/j.1365-2672.2008.03860.x. Epub 2008 Sep 5.
To determine the antioxidative activity, glutathione production, acid and bile tolerance and carbohydrate preferences of Lactobacillus plantarum LP 1, Streptococcus thermophilus Z 57 and Bifidobacterium lactis B 933.
The intact bacteria exhibited antioxidative capacity against linolenic acid and ascorbate oxidation. The antioxidative activity of cell-free extracts was determined by chemiluminescent assay and agreed with total glutathione content. Superoxide dismutase was negligible in all the strains. Bile and gastric juice resistance was tested in vitro to estimate the transit tolerance in the upper gastrointestinal tract. Bifidobacterium lactis B 933 and L. plantarum LP 1 were more acid tolerant than S. thermophilus Z 57. All the strains were resistant to bile. Among 13 indigestible carbohydrates, galacto-oligosaccharides and fructo-oligosaccharides were utilized by all the strains and did not affect survival in human gastric juice.
These potential probiotic strains exhibited antioxidative properties and good viability in gastric juice and bile may indicate tolerance to the transit through the upper gastrointestinal tract. Galacto-oligosaccharides and fructo-oligosaccharides are the most appropriate prebiotics to be used in effective synbiotic formulations.
These results outline promising strains with antioxidative properties. Carbohydrate preferences can be exploited in order to develop synbiotic products.
确定植物乳杆菌 LP1、嗜热链球菌 Z57 和乳双歧杆菌 B933 的抗氧化活性、谷胱甘肽生成、耐酸和耐胆汁能力以及碳水化合物偏好。
完整细菌表现出对亚油酸和抗坏血酸氧化的抗氧化能力。细胞提取物的抗氧化活性通过化学发光测定法确定，并与总谷胱甘肽含量一致。所有菌株中超氧化物歧化酶都可以忽略不计。体外测试了胆汁和胃液抗性，以估计在上胃肠道中的转运耐受性。双歧杆菌 B933 和植物乳杆菌 LP1 比嗜热链球菌 Z57 更耐酸。所有菌株均耐胆汁。在 13 种不可消化的碳水化合物中，所有菌株都利用半乳糖低聚糖和果低聚糖，并且不会影响在人胃液中的存活。
这些潜在的益生菌菌株具有抗氧化特性，并且在胃液和胆汁中具有良好的活力，这可能表明它们能够耐受上胃肠道的转运。半乳糖低聚糖和果低聚糖是最适合用于有效共生制剂的益生元。
这些结果概述了具有抗氧化特性的有前途的菌株。可以利用碳水化合物偏好来开发共生产品。
