Danisco Finland, Health and Nutrition, Kantvik, Finland.
Benef Microbes. 2010 Jun;1(2):139-48. doi: 10.3920/BM2009.0029.
The current screening study aimed at identifying promising prebiotic and synbiotic candidates. The fermentation of xylo-oligosaccharides, xylan, galacto-oligosaccharide, fructo-oligosaccharide, polydextrose, lactitol, gentiobiose and pullulan was investigated in vitro. The ability of these established and potential prebiotic candidates to function as a sole carbon source for probiotic (Bifidobacterium and Lactobacillus), intestinal and potential pathogenic microbes (Eubacterium, Bacteroides, Clostridium, Escherichia coli, Salmonella, and Staphylococcus) was assessed in pure cultures. Xylo-oligosaccharides were fermented with high specificity by the tested Bifidobacterium lactis strains and lactitol by lactobacilli, whereas galacto-oligosaccharides, fructo-oligosaccharides and gentiobiose were utilised by a larger group of microbes. Xylan, polydextrose and pullulan were utilised to a limited extent by only a few of the tested microbes. The results of this screening study indicate that xylo-oligosaccharides and lactitol support the growth of a limited number of beneficial microbes in pure cultures. Such a high degree of specificity has not been previously reported for established prebiotics. Based on these results, the most promising prebiotics and synbiotic combinations can be selected for further testing.
当前的筛选研究旨在确定有前途的益生元和合生元候选物。体外研究了木低聚糖、木聚糖、半乳糖低聚糖、果低聚糖、聚右旋糖、乳糖醇、龙胆二糖和普鲁兰的发酵情况。评估了这些已建立和潜在的益生元候选物作为益生菌（双歧杆菌和乳杆菌）、肠道和潜在致病微生物（真杆菌、拟杆菌、梭菌、大肠杆菌、沙门氏菌和葡萄球菌）的唯一碳源的能力。在纯培养物中。测试的双歧杆菌 lactis 菌株对木低聚糖具有很高的特异性发酵，而乳杆菌对乳糖醇具有很高的特异性发酵，而半乳糖低聚糖、果低聚糖和龙胆二糖被更多的微生物利用。木聚糖、聚右旋糖和普鲁兰仅被少数测试微生物有限地利用。这项筛选研究的结果表明，木低聚糖和乳糖醇在纯培养物中支持有限数量的有益微生物的生长。这种高度的特异性以前没有在已建立的益生元中报道过。基于这些结果，可以选择最有前途的益生元和合生元组合进行进一步测试。
